KEGG ENZYME: 1.1.1.329

ENZYME: 1.1.1.329

Entry

EC 1.1.1.329                Enzyme

Name

2-deoxy-scyllo-inosamine dehydrogenase;
neoA (gene name);
kanK (gene name, ambiguous);
kanE (gene name, ambiguous)

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

2-deoxy-scyllo-inosamine:NAD(P)+ 1-oxidoreductase

Reaction(IUBMB)

2-deoxy-scyllo-inosamine + NAD(P)+ = 3-amino-2,3-dideoxy-scyllo-inosose + NAD(P)H + H+ [RN:R08892 R10130]

Reaction(KEGG)

R08892 R10130

Substrate

2-deoxy-scyllo-inosamine [CPD:C17580];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

3-amino-2,3-dideoxy-scyllo-inosose [CPD:C17581];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

Requires zinc. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, neomycin and ribostamycin. cf. EC 1.1.99.38, 2-deoxy-scyllo-inosamine dehydrogenase (AdoMet-dependent).

History

EC 1.1.1.329 created 2012

Pathway

ec00524

Neomycin, kanamycin and gentamicin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13548

2-deoxy-scyllo-inosamine dehydrogenase

Genes

JAV:	OXU80_01100

NIE:	KV110_24020

SRN:	A4G23_05130(neoA)

SFB:	CP974_27375

SOV:	QZH56_04530

DVC:	Dvina_05415

Reference

1 [PMID:16506694]

Authors

Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K

Title

Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.

Journal

J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104

Sequence

[ag:BAD95818]

Reference

2 [PMID:19466609]

Authors

Nepal KK, Oh TJ, Sohng JK

Title

Heterologous production of paromamine in Streptomyces lividans TK24 using kanamycin biosynthetic genes from Streptomyces kanamyceticus ATCC12853.

Journal

Mol Cells 27:601-8 (2009)
DOI:10.1007/s10059-009-0080-5

Other DBs

ExplorEnz - The Enzyme Database:

1.1.1.329

IUBMB Enzyme Nomenclature:

1.1.1.329

ExPASy - ENZYME nomenclature database:

1.1.1.329

BRENDA, the Enzyme Database:

1.1.1.329

DBGET integrated database retrieval system