KEGG ENZYME: 1.1.1.356

ENZYME: 1.1.1.356

Entry

EC 1.1.1.356                Enzyme

Name

GDP-L-colitose synthase;
ColC

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

GDP-beta-L-colitose:NAD(P)+ 4-oxidoreductase (5-epimerizing)

Reaction(IUBMB)

GDP-beta-L-colitose + NAD(P)+ = GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ [RN:R10420 R10421]

Reaction(KEGG)

R10420 R10421

Substrate

GDP-beta-L-colitose [CPD:C20613];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose [CPD:C20612];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The enzyme is involved in biosynthesis of L-colitose, a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides, where it catalyses the reaction in the reverse direction. The enzyme also performs the NAD(P)H-dependent epimerisation at C-5 of the sugar. The enzyme from Yersinia pseudotuberculosis is Si-specific with respect to NAD(P)H [1].

History

EC 1.1.1.356 created 2013

Pathway

ec00541

O-Antigen nucleotide sugar biosynthesis

ec01100

Metabolic pathways

Orthology

K21130

GDP-L-colitose synthase

Genes

YSI:	BF17_13440

AAPC:

QG404_01575

VGI:	MID13_00930

VOG:	LCH97_15630

SNOS:

K8P63_01935

PAUN:

MJA45_22845

MSIL:

METEAL_02500(fcl)

CFAS:

Cfast33896_17320(rfbD_3)

FBQ:	D1817_12925

Reference

1 [PMID:15610039]

Authors

Alam J, Beyer N, Liu HW

Title

Biosynthesis of colitose: expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC).

Journal

Biochemistry 43:16450-60 (2004)
DOI:10.1021/bi0483763

Sequence

[ag:AEP25495]

Other DBs

ExplorEnz - The Enzyme Database:

1.1.1.356

IUBMB Enzyme Nomenclature:

1.1.1.356

ExPASy - ENZYME nomenclature database:

1.1.1.356

BRENDA, the Enzyme Database:

1.1.1.356

DBGET integrated database retrieval system