KEGG ENZYME: 1.1.1.391

ENZYME: 1.1.1.391

Entry

EC 1.1.1.391                Enzyme

Name

3beta-hydroxycholanate 3-dehydrogenase (NAD+);
3beta-hydroxysteroid dehydrogenase

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

isolithocholate:NAD+ 3-oxidoreductase

Reaction(IUBMB)

isolithocholate + NAD+ = 3-oxo-5beta-cholan-24-oate + NADH + H+ [RN:R11144]

Reaction(KEGG)

R11144

Substrate

isolithocholate [CPD:C17658];
NAD+ [CPD:C00003]

Product

3-oxo-5beta-cholan-24-oate [CPD:C03070];
NADH [CPD:C00004];
H+ [CPD:C00080]

Comment

This bacterial enzyme is involved, along with EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+), or EC 1.1.1.392, 3alpha-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 1.1.1.393, 3beta-hydroxycholanate 3-dehydrogenase (NADP+).

History

EC 1.1.1.391 created 2016

Pathway

ec00121

Secondary bile acid biosynthesis

Orthology

K22606

3beta-hydroxycholanate 3-dehydrogenase (NAD+)

Genes

SHAN:

PPZ50_07710

HHJ:	NQ487_00530

ELE:	Elen_0198 Elen_1325

EGD:	GS424_001135 GS424_007065

GPA:

GPA_22420

Reference

1 [PMID:2752021]

Authors

Edenharder R, Pfutzner A, Hammann R

Title

Characterization of NAD-dependent 3 alpha- and 3 beta-hydroxysteroid dehydrogenase and of NADP-dependent 7 beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus.

Journal

Biochim Biophys Acta 1004:230-8 (1989)
DOI:10.1016/0005-2760(89)90272-5

Reference

2 [PMID:2764572]

Authors

Edenharder R, Pfutzner M

Title

Partial purification and characterization of an NAD-dependent 3 beta-hydroxysteroid dehydrogenase from Clostridium innocuum.

Journal

Appl Environ Microbiol 55:1656-9 (1989)
DOI:10.1128/AEM.55.6.1656-1659.1989

Reference

3 [PMID:26192599]