KEGG   ENZYME: 1.1.1.395
Entry
EC 1.1.1.395                Enzyme                                 
Name
3alpha-hydroxy bile acid-CoA-ester 3-dehydrogenase;
baiA1 (gene name);
baiA2 (gene name);
baiA3 (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3alpha-hydroxy-bile-acid-CoA-ester:NAD+ 3-oxidoreductase
Reaction(IUBMB)
a 3alpha-hydroxy bile acid CoA ester + NAD+ = a 3-oxo bile acid CoA ester + NADH + H+
Substrate
3alpha-hydroxy bile acid CoA ester;
NAD+ [CPD:C00003]
Product
3-oxo bile acid CoA ester;
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This bacterial enzyme is involved in the 7-dehydroxylation process associated with bile acid degradation. The enzyme has very little activity with unconjugated bile acid substrates. It has similar activity with choloyl-CoA, chenodeoxycholoyl-CoA, deoxycholoyl-CoA, and lithocholoyl-CoA.
History
EC 1.1.1.395 created 2016
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K15869  3alpha-hydroxy bile acid-CoA-ester 3-dehydrogenase
Genes
CHYL: CE91St63_17730(fabG_2)
Reference
1  [PMID:7766153]
  Authors
Mallonee DH, Lijewski MA, Hylemon PB
  Title
Expression in Escherichia coli and characterization of a bile acid-inducible 3 alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708.
  Journal
Curr Microbiol 30:259-63 (1995)
DOI:10.1007/bf00295498
Reference
2  [PMID:23836456]
  Authors
Bhowmik S, Jones DH, Chiu HP, Park IH, Chiu HJ, Axelrod HL, Farr CL, Tien HJ, Agarwalla S, Lesley SA
  Title
Structural and functional characterization of BaiA, an enzyme involved in secondary bile acid synthesis in human gut microbe.
  Journal
Proteins 82:216-29 (2014)
DOI:10.1002/prot.24353
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.395
IUBMB Enzyme Nomenclature: 1.1.1.395
ExPASy - ENZYME nomenclature database: 1.1.1.395
BRENDA, the Enzyme Database: 1.1.1.395

  All links  
Pathway (2)   
   KEGG PATHWAY (2)   
Gene (3)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
Protein sequence (72)   
   UniProt (70)   
   SWISS-PROT (2)   
DNA sequence (143)   
   GenBank (85)   
   EMBL (58)   
Protein domain (4)   
   InterPro (4)   
All databases (224)   

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