Entry
Name
2-glutathionyl-2-methylbut-3-en-1-ol dehydrogenase;
isoH (gene name);
4-hydroxy-3-glutathionyl-3-methylbut-1-ene dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol:NAD+ oxidoreductase
Reaction(IUBMB)
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol + 2 NAD+ + H2O = 2-(glutathion-S-yl)-2-methylbut-3-enoate + 2 NADH + 2 H+ (overall reaction);
(1a) 2-(glutathion-S-yl)-2-methylbut-3-en-1-ol + NAD+ = 2-(glutathion-S-yl)-2-methylbut-3-enal + NADH + H+;
(1b) 2-(glutathion-S-yl)-2-methylbut-3-enal + NAD+ + H2O = 2-(glutathion-S-yl)-2-methylbut-3-enoate + NADH + H+
Substrate
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol [CPD:
C21374 ];
NAD+ [CPD:
C00003 ];
H2O [CPD:
C00001 ];
2-(glutathion-S-yl)-2-methylbut-3-enal
Product
2-(glutathion-S-yl)-2-methylbut-3-enoate;
NADH [CPD:
C00004 ];
H+ [CPD:
C00080 ];
2-(glutathion-S-yl)-2-methylbut-3-enal
Comment
The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation.
History
EC 1.1.1.398 created 2016
Orthology
K21101 2-glutathionyl-2-methylbut-3-en-1-ol dehydrogenase
Genes
Taxonomy
Reference
Authors
van Hylckama Vlieg JE, Kingma J, Kruizinga W, Janssen DB
Title
Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 1.1.1.398