KEGG   ENZYME: 1.1.1.418
Entry
EC 1.1.1.418                Enzyme                                 
Name
plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating);
3beta-HSD/D1 (gene name);
3beta-HSD/D2 (gene name);
3beta-hydroxysteroid dehydrogenases/C-4 decarboxylase (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3beta-hydroxysteroid-4alpha-carboxylate:NAD+ 3-oxidoreductase (decarboxylating)
Reaction(IUBMB)
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+ = a 3-oxosteroid + CO2 + NADH [RN:R12366]
Reaction(KEGG)
R12366 > R12371 R12375 R12376
Substrate
3beta-hydroxysteroid-4alpha-carboxylate [CPD:C22111];
NAD+ [CPD:C00003]
Product
3-oxosteroid [CPD:C01876];
CO2 [CPD:C00011];
NADH [CPD:C00004]
Comment
The enzyme, found in plants, catalyses multiple reactions during plant sterol biosynthesis. Unlike the fungal/animal enzyme EC 1.1.1.170, 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating), the plant enzyme is specific for NAD+.
History
EC 1.1.1.418 created 2019
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23558  plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase
Genes
MMER: 123543975
ATH: AT1G47290(3BETAHSD/D1) AT2G26260(3BETAHSD/D2) AT2G43420
ALY: 9315018 9317969 9327409
CRB: 17888910 17889700 17898596
CSAT: 104703154 104714089 104752257 104758028 104777665 104782858 104785013 104793273 109124732
EUS: EUTSA_v10001381mg EUTSA_v10001949mg EUTSA_v10011495mg
BRP: 103842157 103843901 103866715
BNA: 106368540 106371310 106390851 106402151 106414952 106421095
BOE: 106296378 106307642 106308495 106341839
RSZ: 108811832 108818777 108856331 108862423 130507773
THJ: 104802316 104803571 104804450 104819910 104819911
CPAP: 110812016 110821405
CIT: 102607843 102626005
CIC: CICLE_v10007884mg CICLE_v10019433mg
PVY: 116117123 116132027 116145720
MINC: 123194436 123206420 123221140
TCC: 18588175 18602449
GRA: 105766177 105773475 105778202 105796377
GHI: 107896916 107901017 107903885 107914644 107927476 107938719 107957226 107961633
GAB: 108476304 108483574 108488634
HSYR: 120130408 120147435 120160927 120182269 120201117 120212770 120218149
DZI: 111279431 111285384 111303015 111307734
EGR: 104435786 104436360
GMX: 100794808 100799363 100800800 100809319 100814147 100815785 100816854
GSJ: 114374173 114379749 114405678 114410541 114410545 114414874 114418722
PVU: PHAVU_009G084200g PHAVU_011G053200g
VRA: 106756699 106760856 106776089
VAR: 108330047 108340625
VUN: 114164232 114170010
VUM: 124838479 124848767
CCAJ: 109791368 109801541
APRC: 113864705 113865991
MTR: 11412484 11426731
TPRA: 123897995 123916902
CAM: 101494810 101500996
PSAT: 127087836 127100543
VVO: 131594855 131594858 131602070 131633737 131653555 131653556 131655201 131655203
LJA: Lj0g3v0192789.1(Lj0g3v0192789.1) Lj2g3v0856600.1(Lj2g3v0856600.1) Lj3g3v3325980.1(Lj3g3v3325980.1)
ADU: 107470664 107470953 107476756
AIP: 107620898 107623291 107628972
AHF: 112716044 112716215 112736795 112783352 112783971 114927374
LANG: 109325732 109328420 109341751 109343798
PCIN: 129298404 129302399
QSA: O6P43_015823 O6P43_019160 O6P43_028093
FVE: 101292864
RCN: 112171861 112199072
PPER: 18791941 18793760
PMUM: 103320667 103322301
PAVI: 110749482 110773108
PDUL: 117614277 117614519
MDM: 103419184 103440612
MSYL: 126602484 126619329 126631752
PXB: 103933227 103949660 103953014
ZJU: 107405093 107420046
MNT: 21384233 21399197 21401592
CSAV: 115720623 115723106
CSV: 101205275 101212494
CMO: 103483967 103488530
BHJ: 120075097 120085819
MCHA: 111004984 111009273
CMAX: 111470875 111493953
CMOS: 111439089 111450770
CPEP: 111801021 111805666
RCU: 8258610 8287636
JCU: 105628414 105638060
HBR: 110639226 110662259
MESC: 110604012 110613045
POP: 18100844 7492281
PEU: 105113088 105116056 105122098
PALZ: 118030630 118032037
JRE: 108985075 108990435
CILL: 122307107 122313728
CAVE: 132166931 132181271
QSU: 111989557 112029405
QLO: 115952975 115963676 115991809
TWL: 119988085 119997351
VVI: 100245662 100257631
VRI: 117913150 117915011
SLY: 101249150 101254144(HSD1) 101256443(HSD2)
SPEN: 107005180 107011682 107011815
SOT: 102587792 102596460 102598251
SSTN: 125848854 125849035 125863127
SDUL: 129870237 129872276 129872894
CANN: 107857986 107859693 107863753
LBB: 132606295 132621415 132636065
NTA: 107780439 107795088 107795373 107814161 107817215 107818175
NSY: 104216879 104239655 104245967
NTO: 104084496 104092502 104108740
NAU: 109210739 109213017 109227010
INI: 109161741 109184677
ITR: 116017169 116025787
SIND: 105164848 105165847 105172010
OEU: 111371187 111377242 111377243 111393378
EGT: 105953922 105967180 105975202
SSPL: 121744496 121745522 121775340 121776174 121778525 121780910 121789729 121790191 121811147
SMIL: 130999868 131004841 131015149 131024937
SHIS: 125186913 125210984 125216741 125219962
APAN: 127241691 127243522 127256008
HAN: 110899855 110908422 110917497 110920605
ECAD: 122587238 122600031 122606208 122608089
LSV: 111875990 111883224 111899990 111919546
CCAV: 112503045 112508871 112511529 112522090
DCR: 108197059 108197988 108203699 108217750 108223476
CSIN: 114279462 114310008 114313733 114320505
RVL: 131301277 131319093
AEW: 130757565 130774034 130776512 130781978 130789931
BVG: 104888851 104904629
SOE: 110787809 110802274
CQI: 110682749 110696539 110717644 110738779
ATRI: 130810042 130812791 130825301
MOF: 131150798 131150961
NNU: 104588912 104605277
MING: 122089108 122093861
TSS: 122640370 122656788
PSOM: 113297813 113300464 113301315 113328380 113348744
OSA: 4333070 4333071 4347580
DOSA: Os03t0404800-01(Os03g0404800) Os03t0405000-01(Os03g0405000) Os09t0516500-00(Os09g0516500)
OBR: 102699988 102711055 102711335
OGL: 127767990 127768251 127785501
BDI: 100832384 100838723 100841751
ATS: 109751859 109759047 109759249 109776475 109780969 109782739
TDC: 119285843 119296052 119296770 119299834 119304981 119311150 119324550 119332326 119339590
TAES: 123085918 123091921 123097331 123104417 123106270 123112715 123119045 123122193 123124996 123141346 123149698 123158639 123161387
TUA: 125529639 125550654
LPER: 127294857 127301852 127302434 127319506 127330772 127332432 127335732 127348623
LRD: 124655051 124655997 124656977 124657335 124669298 124670959 124671193 124671537 124671610 124680552 124696556 124697677 124702626 124702629 124704686
SBI: 110431777 110431804 8063889 8077068 8077069 8077070 8077071 8077107 8077112
ZMA: 100283692 100383338 103647586
SITA: 101762396 101776881 101778928 101779594 101779992
SVS: 117835670 117836785 117836879 117836880 117836994 117838718 117846541
PVIR: 120652105 120652106 120661244 120684071 120690955
PHAI: 112877458 112881953
PDA: 103702195 103706039 120103893
EGU: 105035203 105056704
MUS: 103968507 103992669
ZOF: 122001417 122005708 122020006 122023907
DCT: 110109494 110113291
PEQ: 110038378 110039408
AOF: 109829223 109838803 109844846
MSIN: 131246402 131251702
NCOL: 116246422 116248785 116267192
ATR: 18431743 18433647 18448731
SMO: SELMODRAFT_129445 SELMODRAFT_84229
PPP: 112282407
 » show all
Reference
1  [PMID:10356290]
  Authors
Rondet S, Taton M, Rahier A
  Title
Identification, characterization, and partial purification of 4 alpha-carboxysterol-C3-dehydrogenase/ C4-decarboxylase from Zea mays.
  Journal
Arch Biochem Biophys 366:249-60 (1999)
DOI:10.1006/abbi.1999.1218
Reference
2  [PMID:16835224]
  Authors
Rahier A, Darnet S, Bouvier F, Camara B, Bard M
  Title
Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana.
  Journal
J Biol Chem 281:27264-77 (2006)
DOI:10.1074/jbc.M604431200
  Sequence
[ath:AT1G47290 AT2G26260]
Reference
3  [PMID:19218365]
  Authors
Rahier A, Bergdoll M, Genot G, Bouvier F, Camara B
  Title
Homology modeling and site-directed mutagenesis reveal catalytic key amino acids of 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase from Arabidopsis.
  Journal
Plant Physiol 149:1872-86 (2009)
DOI:10.1104/pp.108.132282
  Sequence
[ath:AT1G47290]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.418
IUBMB Enzyme Nomenclature: 1.1.1.418
ExPASy - ENZYME nomenclature database: 1.1.1.418
BRENDA, the Enzyme Database: 1.1.1.418
CAS: 71822-23-6

  All links  
Pathway (7)   
   KEGG PATHWAY (6)   
   KEGG MODULE (1)   
Chemical substance (12)   
   KEGG COMPOUND (12)   
Chemical reaction (6)   
   KEGG REACTION (4)   
   KEGG RCLASS (2)   
Gene (507)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (482)   
   KEGG MGENES (24)   
Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (4)   
   InterPro (4)   
All databases (540)   

Download RDF
DBGET integrated database retrieval system