KEGG ENZYME: 1.1.1.436

ENZYME: 1.1.1.436

Entry

EC 1.1.1.436                Enzyme

Name

lactate dehydrogenase (NAD+,ferredoxin);
electron bifurcating LDH/Etf complex

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

lactate:NAD+,ferredoxin oxidoreductase

Reaction(IUBMB)

lactate + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster = pyruvate + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R11676]

Reaction(KEGG)

R11676 > R13049 R13050

Substrate

lactate [CPD:C01432];
NAD+ [CPD:C00003];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138]

Product

pyruvate [CPD:C00022];
NADH [CPD:C00004];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]

Comment

The enzyme, isolated from the bacterium Acetobacterium woodii, uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+.

History

EC 1.1.1.436 created 2015 as EC 1.3.1.110, transferred 2022 to EC 1.1.1.436

Pathway

ec00620

Pyruvate metabolism

ec01100

Metabolic pathways

Orthology

K26113

lactate dehydrogenase (NAD+,ferredoxin) subunit LctD

Genes

GAP:	GAPWK_1784

GAQ:	RAM11_05485

PSAB:

PSAB_09495

PDU:	PDUR_18785

PSOP:

KP014_23470

ALOY:

CJ190_000015

AMIT:

DBT49_0000015

ATJ:	DBT50_000015

FCT:	NRE15_03860

CAC:

CA_C2542

CAE:	SMB_G2577(glcD)

CAY:

CEA_G2555

CPE:

CPE0313

CPF:

CPF_0310

CPR:

CPR_0305

CTC:

CTC_00976

CTET:

BN906_01038

CNO:	NT01CX_1612

CBO:	CBO1009(glcD)

CBA:

CLB_1049

CBH:

CLC_1062

CBY:

CLM_1164

CBL:

CLK_0449

CBK:	CLL_A0365 CLL_A1364

CBB:

CLD_3557

CBI:

CLJ_B1054

CBN:

CbC4_0797

CBT:	CLH_0350 CLH_1310

CBF:

CLI_1091

CBM:

CBF_1061

CBJ:	H04402_01082

CBE:	Cbei_0312 Cbei_2884

CBZ:	Cbs_0312 Cbs_2884

CBEI:

LF65_00348 LF65_02571

CLJ:	CLJU_c21570

CLS:	CXIVA_02370

CSR:	Cspa_c03510(glcD1) Cspa_c19410(glcD2) Cspa_c41430

CPAS:

Clopa_3349 Clopa_3664

CPAT:

CLPA_c14970(glcD1) CLPA_c29580(glcD2)

CPAE:

CPAST_c14970(glcD1) CPAST_c29580(glcD2)

CSB:	CLSA_c03500(glcD1) CLSA_c19070(glcD2)

CAH:	CAETHG_0244

CBV:

U729_1781

CSQ:	CSCA_0591 CSCA_2483

CACE:

CACET_c37490(glcD2)

CCK:	Ccar_02285 Ccar_11415

CBUT:

ATN24_03035 ATN24_08895 ATN24_11095

CTYK:

CTK_C13670 CTK_C15710 CTK_C26210

CEU:	A7L45_05755 A7L45_05810

CTAE:

BGI42_01635

CFM:	BJL90_04460

CCHV:

BTM20_02535

CDRK:

B9W14_07280 B9W14_15575

CIA:	BEN51_10830

CSEP:

CP523_05835

CDY:	F3K33_01575 F3K33_14625

CCOH:

SAMEA4530647_0782

CFER:

D4Z93_07470

CGAS:

J1C67_18485

CLOS:

DMR38_11125

CCAA:

KQH81_05590 KQH81_16525

CMAH:

C1I91_21590

CRW:	CROST_032090 CROST_034820

CFB:	CLFE_013700 CLFE_032010

CAUN:

CLAUR_041110 CLAUR_044190

CNN:	CNEO_2276 CNEO_2611

CGEL:

psyc5s11_17870 psyc5s11_17920 psyc5s11_17980(glcD_1) psyc5s11_21450(glcD_2)

CTAG:

LL095_05935

CINT:

HZF06_09775 HZF06_19080

CTHD:

CDO33_12595

CPRF:

K7H06_11775

SARJ:

HH195_01215

RBP:	B6259_01780

RUM:

CK1_25560

CLF:	GJQ69_08645

CARL:

PXC00_01410

ACOL:

K5I23_04370

PETR:

QKW49_19745

CAPR:

EQM14_05780 EQM14_11115

CFEM:

HCR03_18985

DOR:	Desor_4125

DAI:	Desaci_0266

DMI:	Desmer_3556

ELM:

ELI_1953

EMT:	CPZ25_002245

ELIM:

B2M23_16670

AWO:	Awo_c08730(glcD)

AWI:	CHL1_002907

ACAE:

HYG86_03160

IBU:	IB211_02524

BPRS:

CK3_30750

CCT:	CC1_07810 CC1_16380

BMAS:

LK422_15540

CPY:	Cphy_1234 Cphy_1317

LXA:	OW255_00695 OW255_07590 OW255_07625 OW255_10930

BPRL:

CL2_13250 CL2_23160 CL2_23560

ARF:	AR1Y2_0863 AR1Y2_1968 AR1Y2_1979

ACAC:

EYQ97_07920 EYQ97_07955 EYQ97_12845

EHL:

EHLA_0974

ACHT:

bsdcttw_20890(glcD)

CBOL:

CGC65_07940

WHJ:	H9Q79_07170

CLE:	Clole_4165

CEW:	EKH84_02325

AMT:

Amet_0821

GFE:	Gferi_16040

PBIF:

KXZ80_10385

PSOR:

RSJ16_12220

CDF:	CD630_11730

PDC:	CDIF630_01321

CDC:	CD196_1032

CDL:	CDR20291_1010

PDF:	CD630DERM_11730

ROC:	HF520_09650

TEM:	JW646_00635

TGC:	L0P85_07705

TMY:	TEMA_15040

TEB:	T8CH_1817(glcD)

THYR:

P4S50_08235

IBR:	NQ514_06205

AAR:	Acear_1804

APR:

Apre_1357

AVG:	I6H45_02075

AOB:	I6H46_05105

PHAR:

NCTC13077_01421

AMEI:

LV469_08110

SSG:	Selsp_1929

SRI:	SELR_16970

MHG:

MHY_15050

MFUN:

GXM21_04085

AFN:	Acfer_0244

AIN:	Acin_2227(glcD)

FRO:	AALO17_25560

FCZ:	IMF26_09255

AOC:	Aocu_12000

AHK:	NCTC10172_00846

ABRA:

BN85303760(glcD)

MANR:

MPAN_017300(glcD)

TBZ:	BK011_10305

OLO:	ADJ70_09920

CCU:	Ccur_10360

SHI:	Shel_12710

DDT:	AAY81_08990

VBC:	C5Q97_07845

TRI:	DYE50_04195

TPER:

IWA51_01090

FNU:

FN1536

FNC:	HMPREF0946_01218

FNT:	HMPREF0405_00180

FUS:	HMPREF0409_01700

FNE:	FSDG_01649

FGO:	C4N16_03545

FNF:	BSQ88_01895

FHO:	H9Q81_00365

FPOL:

ERS445057_02142

IPO:	Ilyop_0987

ALAM:

RT761_00853

» show all

Reference

1 [PMID:24762045]

Authors

Weghoff MC, Bertsch J, Muller V

Title

A novel mode of lactate metabolism in strictly anaerobic bacteria.

Journal

Environ Microbiol 17:670-7 (2015)
DOI:10.1111/1462-2920.12493

Sequence

[awo:Awo_c08710 Awo_c08720 Awo_c08730]

Other DBs

ExplorEnz - The Enzyme Database:

1.1.1.436

IUBMB Enzyme Nomenclature:

1.1.1.436

ExPASy - ENZYME nomenclature database:

1.1.1.436

BRENDA, the Enzyme Database:

1.1.1.436

DBGET integrated database retrieval system