KEGG ENZYME: 1.1.3.47

ENZYME: 1.1.3.47

Entry

EC 1.1.3.47                 Enzyme

Name

5-(hydroxymethyl)furfural oxidase

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor

Sysname

5-(hydroxymethyl)furfural:oxygen oxidoreductase

Reaction(IUBMB)

5-(hydroxymethyl)furfural + 3 O2 + 2 H2O = furan-2,5-dicarboxylate + 3 H2O2 (overall reaction) [RN:R10858];
(1a) 5-(hydroxymethyl)furfural + O2 = furan-2,5-dicarbaldehyde + H2O2 [RN:R10853];
(1b) furan-2,5-dicarbaldehyde + H2O = 5-(dihydroxymethyl)furan-2-carbaldehyde (spontaneous) [RN:R10854];
(1c) 5-(dihydroxymethyl)furan-2-carbaldehyde + O2 = 5-formylfuran-2-carboxylate + H2O2 [RN:R10855];
(1d) 5-formylfuran-2-carboxylate + H2O = 5-(dihydroxymethyl)furan-2-carboxylate (spontaneous) [RN:R10856];
(1e) 5-(dihydroxymethyl)furan-2-carboxylate + O2 = furan-2,5-dicarboxylate + H2O2 [RN:R10857]

Reaction(KEGG)

R10853 R10854 R10855 R10856 R10857 R10858

Substrate

5-hydroxymethylfurfural [CPD:C11101];
O2 [CPD:C00007];
H2O [CPD:C00001];
furan-2,5-dicarbaldehyde [CPD:C20899];
5-(dihydroxymethyl)furan-2-carbaldehyde [CPD:C20900];
5-formylfuran-2-carboxylate [CPD:C20449];
5-(dihydroxymethyl)furan-2-carboxylate [CPD:C20901]

Product

furan-2,5-dicarboxylate [CPD:C20450];
H2O2 [CPD:C00027];
furan-2,5-dicarbaldehyde [CPD:C20899];
5-(dihydroxymethyl)furan-2-carbaldehyde [CPD:C20900];
5-formylfuran-2-carboxylate [CPD:C20449];
5-(dihydroxymethyl)furan-2-carboxylate [CPD:C20901]

Comment

The enzyme, characterized from the bacterium Methylovorus sp. strain MP688, is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural. The enzyme acts only on alcohol groups and requires the spontaneous hydration of aldehyde groups for their oxidation [3]. The enzyme has a broad substrate range that overlaps with EC 1.1.3.7, aryl-alcohol oxidase.

History

EC 1.1.3.47 created 2014

Pathway

ec00365

Furfural degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K16873

5-(hydroxymethyl)furfural/furfural oxidase

Genes

PWI:	MWN52_02930

PMUI:

G4G71_01325

PNT:	G5B91_01695

PSK:	U771_11545

PRH:	LT40_15490

PPAO:

K3169_17575

HAK:	KO116_01524

CNC:	CNE_BB2p00120(betA)

CUH:	BJN34_06490 BJN34_36300

BCJ:

BCAL1185

BCEN:

DM39_4075

BAEN:

L3V59_43085

BUG:	BC1001_3784 BC1001_5246

BGF:	BC1003_0595 BC1003_4169

BGD:	bgla_2g10080

BGO:

BM43_4645

BYI:	BYI23_C009090

BUE:	BRPE67_CCDS02070

BUQ:	AC233_23790

BUM:	AXG89_18100 AXG89_39615

BUI:	AX768_20745 AX768_27875

BXE:

Bxe_B1504

BXB:

DR64_6811

BPH:	Bphy_6576 Bphy_6668

BGE:	BC1002_5742

BPX:	BUPH_00358 BUPH_05836

BPY:	Bphyt_2180 Bphyt_6155

BFN:	OI25_3193 OI25_5052

BCAI:

K788_0001302 K788_0006280

PARB:

CJU94_31765 CJU94_33585

PHS:	C2L64_36945 C2L64_39405

PTER:

C2L65_32445 C2L65_44890

PGP:	CUJ91_19755

PCJ:	CUJ87_08665 CUJ87_25040

PTS:	CUJ90_26195

PTRO:

G5S35_29105

PDIO:

PDMSB3_1602 PDMSB3_1606.1 PDMSB3_1611

PSAA:

QEN71_31975 QEN71_39395

PKF:	RW095_17780 RW095_28670

PPAI:

E1956_41815 E1956_41860

CABA:

SBC2_51220

CABK:

NK8_63750 NK8_78640

TCAR:

U0034_12350

BFZ:	BAU07_17215

AIS:	BUW96_05150

AMIM:

MIM_c00530

ODI:

ODI_R0536

PIG:	EGT29_10250 EGT29_25850

MVAR:

MasN3_29710

MEI:	Msip34_0111

MEP:	MPQ_0130(betA)

MLN:	A9174_31955 A9174_32170

MCIC:

A4R28_21725 A4R28_21785

MJR:	EB229_32675 EB229_32880

MERD:

EB233_26695 EB233_30565

MMEI:

LRP31_10170

MESO:

BSQ44_09535

SINO:

SS05631_a47220 SS05631_a47520

ATF:	Ach5_47410

ALEG:

CFBP4996_20540

RLT:	Rleg2_4996

RJG:	CCGE525_35685 CCGE525_35845

RLS:	HB780_12845

RAW:	NE851_07995

ARA:

Arad_7048

SOJ:	K6301_24775

SSUM:

Q9314_23445

OCH:	CES85_3113

BJA:	blr0367(blr0367)

BJU:	BJ6T_25830 BJ6T_61910

BJP:	RN69_12590 RN69_29990

BRS:

S23_14520

BRC:	BCCGELA001_16395

BRAD:

BF49_1118

BOT:	CIT37_34325

BRQ:	CIT40_12415

BGQ:	X265_02415 X265_16195

BGZ:	XH91_03285

BSYM:

CIT39_11665

BBET:

F8237_22615

BARH:

WN72_17985 WN72_21175

BVZ:	BRAD3257_5110

BDG:	LPJ38_21810

BBAN:

J4G43_042000

BCAN:

BcanWSM471_17045

BXN:	I3J27_29665

BROS:

QUH67_09490

BOF:	FQV39_10330 FQV39_30935

STAR:

G3545_26520

LNE:	FZC33_06990

APRA:

G3A50_21195

APOL:

K9D25_00990 K9D25_03940 K9D25_23365

MRD:	Mrad2831_4733

MET:

M446_1311

MNO:

Mnod_4787

MOR:

MOC_3629

MAQU:

Maq22A_2p41610(betA) Maq22A_c13940(betA)

MPHY:

MCBMB27_05482

MEE:	DA075_33415

METX:

A3862_05035

MIND:

mvi_56510

RHZ:	RHPLAN_54060

DEQ:	XM25_21010

DEA:	FPZ08_18070

YTI:	FNA67_02335

MHAR:

L1P08_14680

HDI:	HDIA_0814(betA_1)

AUA:	M673_12200

AALA:

IGS74_03065

AALM:

LUX29_03900

PSTG:

E8M01_20935

RID:	RIdsm_02758(betA_4)

RPEL:

N7U68_13165

HAT:	RC74_03320

PYE:	A6J80_21445

PPAN:

ESD82_07655

PMEH:

JWJ88_18740

PPRU:

FDP22_00465

PAED:

G5B38_10760

NRE:	BES08_14155

SWI:

Swit_4242

RDI:	CMV14_05195

SKR:	BRX40_00090

SNAP:

PQ455_04425

SCH:	Sphch_3353

ACEK:

FLP30_09580

RFL:

Rmf_16260

SRF:	LHU95_17970

ACTQ:

OG417_12875

» show all

Reference

1 [PMID:20194784]

Authors

Koopman F, Wierckx N, de Winde JH, Ruijssenaars HJ

Title

Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14.

Journal

Proc Natl Acad Sci U S A 107:4919-24 (2010)
DOI:10.1073/pnas.0913039107

Sequence

[ag:ADE20408]

Reference

2 [PMID:24271187]

Authors

Dijkman WP, Fraaije MW

Title

Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688.

Journal

Appl Environ Microbiol 80:1082-90 (2014)
DOI:10.1128/AEM.03740-13

Sequence

[mep:MPQ_0130]

Reference

3 [PMID:24802551]

Authors

Dijkman WP, Groothuis DE, Fraaije MW

Title

Enzyme-catalyzed oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid.

Journal

Angew Chem Int Ed Engl 53:6515-8 (2014)
DOI:10.1002/anie.201402904

Other DBs

ExplorEnz - The Enzyme Database:

1.1.3.47

IUBMB Enzyme Nomenclature:

1.1.3.47

ExPASy - ENZYME nomenclature database:

1.1.3.47

BRENDA, the Enzyme Database:

1.1.3.47

DBGET integrated database retrieval system