KEGG ENZYME: 1.1.9.1

ENZYME: 1.1.9.1

Entry

EC 1.1.9.1                  Enzyme

Name

alcohol dehydrogenase (azurin);
type II quinoprotein alcohol dehydrogenase;
quinohaemoprotein ethanol dehydrogenase;
QHEDH;
ADHIIB

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With a copper protein as acceptor

Sysname

alcohol:azurin oxidoreductase

Reaction(IUBMB)

a primary alcohol + azurin = an aldehyde + reduced azurin [RN:R09480]

Reaction(KEGG)

R09480

Substrate

primary alcohol [CPD:C00226];
azurin

Product

aldehyde [CPD:C00071];
reduced azurin [CPD:C05358]

Comment

A soluble, periplasmic PQQ-containing quinohemoprotein. Also contains a single heme c. Occurs in Comamonas and Pseudomonas. Does not require an amine activator. Oxidizes a wide range of primary and secondary alcohols, and also aldehydes and large substrates such as sterols; methanol is not a substrate. Usually assayed with phenazine methosulfate or ferricyanide. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ.

History

EC 1.1.9.1 created 2010 as EC 1.1.98.1; transferred 2011 to EC 1.1.9.1

Orthology

K17760

quinohemoprotein ethanol dehydrogenase

Genes

PMK:

MDS_2680

PCQ:	PcP3B5_33080(qgdA_1)

PMUI:

G4G71_16070

PPW:	PputW619_2696

PSK:	U771_15535

PPSY:

AOC04_06000

PUM:	HGP31_10690

POJ:	PtoMrB4_39170

PXA:	KSS93_18230

PQI:	KH389_15205 KH389_15915 KH389_17020

PSHH:

HU773_015410

PASG:

KSS96_11385

PFAK:

KSS94_13150

PALV:

KSS97_17250

PTW:	TUM18999_41850

PTAE:

NCTC10697_01924(qheDH)

PCAV:

D3880_11910

PHF:	NLY38_13805

PPHN:

HU825_14525 HU825_15730

PIZ:	LAB08_R21640

PCAS:

LOY40_16600 LOY40_17405 LOY40_17685

PKM:	PZ739_13820

PSOA:

PSm6_02790

PSZ:	PSTAB_2905

PSR:	PSTAA_3020

PSH:	Psest_1423

PSTT:

CH92_09640

PSTU:

UIB01_10105

PSED:

DM292_04320 DM292_07550

PCHL:

LLJ08_06255 LLJ08_09455

PBM:	CL52_14245

PKG:	LW136_16105

SDEG:

GOM96_01520 GOM96_02720

PZD:	KQ248_10230

PBB:	AKN87_00160

HPEG:

EAO82_13780

HAES:

LO767_05585

PDEN:

F1C79_07700

HARR:

HV822_16280

PNN:	KEM63_08795

MSR:	AU15_01600

MARI:

ACP86_11840

MLQ:	ASQ50_13195

MARJ:

MARI_09920(qheDH)

MSHE:

MAALD49_01730

MANP:

EHN06_00340

PSPI:

PS2015_1348 PS2015_2383

SALK:

FBQ74_01340

GPS:

C427_3776

SPOI:

IMCC21906_02691

SNAN:

I6N98_04765 I6N98_07195 I6N98_13860

ZAL:	AZF00_00410 AZF00_02915 AZF00_06825

OSG:	BST96_06520 BST96_11945 BST96_14245 BST96_17020

HJA:	BST95_15490

HALC:

EY643_01600

KIM:	G3T16_00650 G3T16_16955

AFUS:

EYZ66_01320 EYZ66_01385 EYZ66_03410 EYZ66_03590 EYZ66_06330

PAQM:

E0F26_08550

ALCA:

ASALC70_00253(qgdA) ASALC70_03961(qheDH)

ADI:	B5T_04379(exaA1)

AXE:

P40_21330

SDF:	ACG33_03485

SOK:	D0B54_04380 D0B54_04385

FCE:	JN531_004925 JN531_005045

REH:	H16_A1884(h16_A1884)

CNC:	CNE_1c18660(qheDH) CNE_BB1p04310(adh)

CUH:	BJN34_09985

REU:	Reut_A1461 Reut_B4160

CTI:	RALTA_A1577(exaA1)

CBW:	RR42_s2370

CCUP:

BKK81_18975 BKK81_27650

CUP:	BKK80_22965 BKK80_28675

CUU:	BKK79_28240 BKK79_34625

COX:	E0W60_10280

CUK:	KB879_21740

CNAN:

A2G96_11225 A2G96_12165

AMIM:

MIM_c00090(qheDH1)

POL:

Bpro_5302

VEI:

Veis_0427

DAC:

Daci_3352

DEL:	DelCs14_3417

DTS:	BI380_02445

DHK:	BO996_15355

DLA:	I6G47_06900 I6G47_09325

VAP:	Vapar_5018

VPE:	Varpa_5729

VPD:	VAPA_1c52090

VBO:	CKY39_31100

VAM:	C4F17_20615

CTT:	CtCNB1_0197 CtCNB1_1388

CTES:

O987_00960

CRJ:	QMY55_01000

OTK:	C6570_08100

HYB:

Q5W_16030

HPSE:

HPF_13145(qheDH)

HYN:	F9K07_23870

HCZ:	G9Q37_12210

PBH:	AAW51_3568(qheDH)

MPT:	Mpe_A0877 Mpe_A0905

METP:

C1M51_13570 C1M51_13725

ATER:

MW290_13615 MW290_24600

DOE:	DENOEST_0490(qbdA) DENOEST_2039(qbdA) DENOEST_3548(qbdA) DENOEST_3675(qbdA)

MBAC:

BN1209_0896

MEU:	ACJ67_07920

AZO:	azo3022(exaA4)

AOA:

dqs_3157

AZR:	CJ010_18755 CJ010_22255

THU:	AC731_008560

THAU:

C4PIVTH_4250(qbdA)

TAK:	Tharo_3323

NIY:	FQ775_00490

PLA:

Plav_1306

PMOB:

HG718_13715

PECT:

BN1012_Phect1521

RBS:	RHODOSMS8_03066(qbdA)

BJU:	BJ6T_73570

BBT:

BBta_5502

BIC:	LMTR13_35850

BRK:	CWS35_13515

BSYM:

CIT39_22055

BVZ:	BRAD3257_1848(qbdA)

BSEI:

KMZ68_24155

BBAN:

J4G43_013560

BAUT:

QA635_04320

BROS:

QUH67_32165

RPA:	TX73_016490

RPB:

RPB_2355

RPD:

RPD_3110

RPE:

RPE_3525

RPT:

Rpal_3601

RPX:	Rpdx1_2242

RBK:	E0H22_16105

VGO:	GJW-30_1_04338(qheDH)

TRB:	HB776_05770

TALZ:

RPMA_12195

XDI:	EZH22_02215

HMC:	HYPMC_4861(adh)

PSIN:

CAK95_13895

RBM:

TEF_09160

LABP:

FJ695_24050

CMB:	CSW64_14395

CAUL:

KCG34_00855 KCG34_05195

PZU:

PHZ_c3112

PHB:	HYN04_10065

TSV:	DSM104635_00613(qgdA)

CID:

P73_2982

THAA:

CFI11_17745

THAS:

C6Y53_11405

RHOC:

QTA57_02650

HNE:	HNE_0129(adhA)

HBC:	AEM38_01780

NAR:	Saro_2870 Saro_2878

NPP:	PP1Y_AT11408 PP1Y_AT11547 PP1Y_Mpl5286

NPN:	JI59_21485

NRE:	BES08_11360 BES08_19965 BES08_22630 BES08_27330

NOV:	TQ38_020540 TQ38_024820

NOT:	C7W88_07620 C7W88_07660

NOR:	FA702_09260 FA702_10240 FA702_20765

NGF:	FRF71_08640

NDR:	HT578_07545 HT578_07615 HT578_09220 HT578_14485

NHUM:

PQ457_02275

SAL:

Sala_0379

SPHK:

SKP52_17900(adh)

SPHP:

LH20_16360

SMAG:

AN936_03210 AN936_16350

SMAZ:

LH19_18005 LH19_18030 LH19_18835

STER:

AOA14_02595

SGI:	SGRAN_3454(adhA2)

SPHQ:

BWQ93_13035

SPHO:

C3E99_12080

SPHX:

E5675_02990

SINA:

KNJ79_14270 KNJ79_15265

SPHU:

SPPYR_0186(qbdA)

SWI:	Swit_0721 Swit_1001 Swit_2227 Swit_5136

SPHD:

HY78_14390 HY78_21510 HY78_23090

RDI:	CMV14_16905 CMV14_19410

SPHI:

TS85_07850

SPKC:

KC8_09425

SPHC:

CVN68_17415

SLAN:

GV829_11830

SSUA:

FPZ54_18055

SBIN:

SBA_ch2_6170 SBA_ch2_6490

SRAD:

LLW23_04025

SNAP:

PQ455_02615 PQ455_04140

SJP:	SJA_C1-02010 SJA_C1-02070 SJA_C2-04000

SINB:

SIDU_17200 SIDU_17230

SCH:	Sphch_0592 Sphch_0603 Sphch_2127

SSY:	SLG_05620 SLG_07280 SLG_12260 SLG_12290 SLG_18610 SLG_20920

SYB:	TZ53_16615 TZ53_17030 TZ53_17090

SPHB:

EP837_00323 EP837_00335(exaA) EP837_03710

SPHR:

BSY17_3920(qbdA) BSY17_934(qbdA)

SPHT:

K426_12160 K426_12220

SHYD:

CJD35_16290 CJD35_16630 CJD35_20620

SYA:	A6768_05740 A6768_05805 A6768_20115

SCLO:

SCLO_1006180 SCLO_1006300

SPYG:

YGS_C2P0050 YGS_C2P0418

SUFL:

FIL70_03770 FIL70_05365 FIL70_05425

SBAR:

H5V43_00800 H5V43_04645 H5V43_05050 H5V43_05110

SAMI:

SAMIE_1016260 SAMIE_1019310

SPPH:

KFK14_04690 KFK14_08020 KFK14_10340 KFK14_13445 KFK14_17040

SPHG:

AZE99_03780 AZE99_04575 AZE99_04655

SFLA:

SPHFLASMR4Y_01514(qbdA)

SPHY:

CHN51_01650

SPHL:

LPB140_10885

SLAA:

EUU25_00640 EUU25_15690

BLAS:

BSY18_3165(adhA) BSY18_3429(qgdA)

BFW:	B5J99_06725 B5J99_07940

SPHJ:

BSL82_14255 BSL82_14300

SPZR:

G5C33_07505

PALG:

HFP57_17830

PARP:

HFP51_01520

PARJ:

J4G78_09460

PHAO:

HF685_12515

ACOB:

P0Y56_05660 P0Y56_09930 P0Y56_12020 P0Y56_13035 P0Y56_15910 P0Y56_16520 P0Y56_16555

SMIC:

SmB9_16520 SmB9_26030(exaA) SmB9_32770

AEP:	AMC99_00019

ALB:	AEB_P1071 AEB_P2933 AEB_P2938

ALH:	G6N82_06175 G6N82_10280

ARUE:

QQX03_03415

AAY:	WYH_01144(qbdA_1) WYH_01921(qbdA_3) WYH_02888(qbdA_4) WYH_03122(qgdA) WYH_03187(qbdA_5)

ADO:	A6F68_00780(qbdA)

EFV:	CHH26_02510

QCI:	NCF85_12430

QSO:	IRL76_11280

QAR:	K3148_07060

QGE:	K3136_06735

QPS:	K3166_11075

ELI:

ELI_14570

ELQ:	Ga0102493_111321

ERK:	CD351_00730

ERR:	DVR09_00745

ERF:	FIU90_00555(qbdA1) FIU90_08125(qbdA2)

EMV:	HQR01_13325

PNS:	A9D12_03105

PORL:

BG023_11102

PHZ:	CHX26_00475

POT:	E2E27_01630

NTD:	EGO55_06120 EGO55_07055

CAEN:

K5X80_12725

ECOG:

FIV45_03450 FIV45_11680

KOZ:	KFF44_04015

AGE:	AA314_07777

BACA:

FAY30_22175

PARH:

I5S86_14975

OCN:	CUC15_15200

LYG:	C1N55_08345

VIM:	GWK91_01760

PSYH:

D0S48_16125

PSYO:

PB01_16660

PSYB:

KD050_07010

NMK:	CHR53_27745

NNV:	QNH39_04635

PAEK:

D3873_10820

AMAV:

GCM10025877_23040

MYL:	C3E77_05855

MUZ:	H4N58_01985

ARHD:

VSH64_08495

AMS:	AMIS_77470

AIH:	Aiant_38910

CWO:

Cwoe_1286

TALB:

FTW19_18675

EDA:	GWR55_07775

ABAC:

LuPra_01740(qgdA_1) LuPra_02092(qbdA_3)

LUB:	TBR22_A09890 TBR22_A16680(exaA_1)

ALEN:

G9X62_03110

IRO:	RT717_16495

CHK:	D4L85_24450

ASAG:

FGM00_10090

» show all

Reference

1 [PMID:3521592]

Authors

Groen BW, van Kleef MA, Duine JA

Title

Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni.

Journal

Biochem J 234:611-5 (1986)
DOI:10.1042/bj2340611

Reference

2 [PMID:7626615]

Authors

de Jong GA, Caldeira J, Sun J, Jongejan JA, de Vries S, Loehr TM, Moura I, Moura JJ, Duine JA

Title

Characterization of the interaction between PQQ and heme c in the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni.

Journal

Biochemistry 34:9451-8 (1995)
DOI:10.1021/bi00029a021

Reference

3 [PMID:7730276]

Authors

Toyama H, Fujii A, Matsushita K, Shinagawa E, Ameyama M, Adachi O

Title

Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols.

Journal

J Bacteriol 177:2442-50 (1995)
DOI:10.1128/JB.177.9.2442-2450.1995

Reference

4 [PMID:10320337]

Authors

Matsushita K, Yamashita T, Aoki N, Toyama H, Adachi O

Title

Electron transfer from quinohemoprotein alcohol dehydrogenase to blue copper protein azurin in the alcohol oxidase respiratory chain of Pseudomonas putida HK5.

Journal

Biochemistry 38:6111-8 (1999)
DOI:10.1021/bi990121f

Reference

5 [PMID:12057198]

Authors

Chen ZW, Matsushita K, Yamashita T, Fujii TA, Toyama H, Adachi O, Bellamy HD, Mathews FS

Title

Structure at 1.9 A resolution of a quinohemoprotein alcohol dehydrogenase from Pseudomonas putida HK5.

Journal

Structure 10:837-49 (2002)
DOI:10.1016/S0969-2126(02)00774-8

Sequence

[ag:BAC15559]

Reference

6 [PMID:11714714]

Authors

Oubrie A, Rozeboom HJ, Kalk KH, Huizinga EG, Dijkstra BW

Title

Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: structural basis for substrate oxidation and electron transfer.

Journal

J Biol Chem 277:3727-32 (2002)
DOI:10.1074/jbc.M109403200

Sequence

[ag:CAA57464]

Other DBs

ExplorEnz - The Enzyme Database:

1.1.9.1

IUBMB Enzyme Nomenclature:

1.1.9.1

ExPASy - ENZYME nomenclature database:

1.1.9.1

BRENDA, the Enzyme Database:

1.1.9.1

DBGET integrated database retrieval system