KEGG ENZYME: 1.1.99.29

ENZYME: 1.1.99.29

Entry

EC 1.1.99.29                Enzyme

Name

pyranose dehydrogenase (acceptor);
pyranose dehydrogenase;
pyranose-quinone oxidoreductase;
quinone-dependent pyranose dehydrogenase;
PDH

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With unknown physiological acceptors

Sysname

pyranose:acceptor oxidoreductase

Reaction(IUBMB)

(1) a pyranose + acceptor = a pyranos-2-ulose (or a pyranos-3-ulose or a pyranos-2,3-diulose) + reduced acceptor [RN:R07347];
(2) a pyranoside + acceptor = a pyranosid-3-ulose (or a pyranosid-3,4-diulose) + reduced acceptor [RN:R07348]

Reaction(KEGG)

R07347 R07348

Substrate

pyranose [CPD:C15592];
acceptor [CPD:C00028];
pyranoside [CPD:C15594]

Product

pyranos-2-ulose;
pyranos-3-ulose;
pyranos-2,3-diulose;
reduced acceptor [CPD:C00030];
pyranosid-3-ulose;
pyranosid-3,4-diulose

Comment

Requires FAD. A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. Unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. D-Glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. Sugars are oxidized in their pyranose but not in their furanose form.

History

EC 1.1.99.29 created 2004

Orthology

K20154

pyranose dehydrogenase

Genes

ABP:	AGABI1DRAFT123150(AGABI1DRAFT_123150)

ABV:	AGABI2DRAFT230189(AGABI2DRAFT_230189)

Reference

1 [PMID:9042751]

Authors

Volc J, Kubatova E, Wood DA, Daniel G.

Title

Pyranose 2-dehydrogenase, a novel sugar oxidoreductase from the basidiomycete fungus Agaricus bisporus

Journal

Arch Microbiol 167:119-25 (1997)

Reference

Authors

Volc, J., Sedmera, P., Halada, P., Přikyrlova, V. and Daniel, G.

Title

C-2 and C-3 oxidation of D-Glc, and C-2 oxidation of D-Gal by pyranose dehydrogenase from Agaricus bisporus.

Journal

Carbohydr Res 310:151-156 (1998)

Reference

3 [PMID:11086703]

Authors

Volc J, Sedmera P, Halada P, Prikrylova V, Haltrich D

Title

Double oxidation of D-xylose to D-glycero -pentos-2,3-diulose (2,3-diketo-D-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus.

Journal

Carbohydr Res 329:219-25 (2000)
DOI:10.1016/S0008-6215(00)00167-1

Sequence

[ag:AAW92124]

Reference

4 [PMID:11511865]

Authors

Volc J, Kubatova E, Daniel G, Sedmera P, Haltrich D.

Title

Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes.

Journal

Arch Microbiol 176:178-86 (2001)
DOI:10.1007/s002030100308

Reference

Authors

Volc, J., Sedmera, P., Halada, P., Daniel, G., Přikyrlova, V. and Haltrich, D.

Title

C-3 oxidation of non-reducing sugars by a fungal pyranose dehydrogenase: spectral characterization.

Journal

J Mol Catal, B Enzym 17:91-100 (2002)

Other DBs

ExplorEnz - The Enzyme Database:

1.1.99.29