The product, L-sorbosone, is an intermediate in bacterial 2-keto-L-gulonic-acid formation. The activity of this membrane-bound enzyme is stimulated by Fe(III) or Co2+ but is inhibited by Cu2+. The enzyme is highly specific for L-sorbose as other sugars, such as glucose, mannitol and sorbitol, are not substrates. Phenazine methosulfate and DCIP can act as artificial acceptors.
History
EC 1.1.99.32 created 2008
Reference
1
Authors
Sugisawa, T., Hoshino, T., Nomura, S. and Fujiwara, A.
Title
Isolation and characterization of membrane-bound L-sorbose dehydrogenase from Gluconobacter melanogenus UV10.