KEGG ENZYME: 1.13.11.74

ENZYME: 1.13.11.74

Entry

EC 1.13.11.74               Enzyme

Name

2-aminophenol 1,6-dioxygenase;
amnA (gene name);
amnB (gene name);
2-aminophenol:oxygen 1,6-oxidoreductase (decyclizing)

Class

Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen

Sysname

2-aminophenol:oxygen 1,6-oxidoreductase (ring-opening)

Reaction(IUBMB)

2-aminophenol + O2 = 2-aminomuconate 6-semialdehyde [RN:R05405]

Reaction(KEGG)

R05405

Substrate

2-aminophenol [CPD:C01987];
O2 [CPD:C00007]

Product

2-aminomuconate 6-semialdehyde [CPD:C03824]

Comment

The enzyme, a member of the nonheme-iron(II)-dependent dioxygenase family, is an extradiol-type dioxygenase that utilizes a non-heme ferrous iron to cleave the aromatic ring at the meta position (relative to the hydroxyl substituent). The enzyme also has some activity with 2-amino-5-methylphenol and 2-amino-4-methylphenol [1]. The enzyme from the bacterium Comamonas testosteroni CNB-1 also has the activity of EC 1.13.11.76, 2-amino-5-chlorophenol 1,6-dioxygenase [2].

History

EC 1.13.11.74 created 2013

Pathway

ec00627

Aminobenzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K15059

2-aminophenol/2-amino-5-chlorophenol 1,6-dioxygenase subunit beta

Genes

PPW:	PputW619_2014

PKC:	PKB_3263(amnb1) PKB_3611(amnb3)

BXE:	Bxe_A1145(amnB)

BXB:	DR64_3304(amnB)

BAV:	BAV0550(amnB)

BHZ:	ACR54_00822(cnbCb)

AXY:	AXYL_02641

AIS:	BUW96_03280

ASW:	CVS48_11345

ACHB:

DVB37_23465

ADY:	HLG70_04300(cnbCb)

APES:

FOC84_32620

AAEG:

RA224_19710(cnbCb)

PIG:	EGT29_21585

NARM:

N7E01_09075(cnbCb)

NHUM:

PQ457_19240 PQ457_19280

SYB:	TZ53_02830

SUFL:

FIL70_13995

SBAR:

H5V43_15190

GSO:	PH603_12425

ARHD:

VSH64_19025

ACTY:

OG774_07860

VMA:	VAB18032_17825

MCRA:

ID554_30935

VER:	HUT12_13600

PRY:	Prubr_10680

SCOR:

J3U87_09070

» show all

Reference

1 [PMID:9169437]

Authors

Takenaka S, Murakami S, Shinke R, Hatakeyama K, Yukawa H, Aoki K

Title

Novel genes encoding 2-aminophenol 1,6-dioxygenase from Pseudomonas species AP-3 growing on 2-aminophenol and catalytic properties of the purified enzyme.

Journal

J Biol Chem 272:14727-32 (1997)
DOI:10.1074/jbc.272.23.14727

Sequence

[ag:BAB03531]

Reference

2 [PMID:15580337]

Authors

Wu JF, Sun CW, Jiang CY, Liu ZP, Liu SJ

Title

A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli.

Journal

Arch Microbiol 183:1-8 (2005)
DOI:10.1007/s00203-004-0738-5

Sequence

[ag:AAT35226]

Reference

3 [PMID:23275161]

Authors

Li de F, Zhang JY, Hou YJ, Liu L, Hu Y, Liu SJ, Wang da C, Liu W

Title

Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor.

Journal

Acta Crystallogr D Biol Crystallogr 69:32-43 (2013)
DOI:10.1107/S0907444912042072