KEGG ENZYME: 1.13.11.76

ENZYME: 1.13.11.76

Entry

EC 1.13.11.76               Enzyme

Name

2-amino-5-chlorophenol 1,6-dioxygenase;
cnbC (gene name);
2-amino-5-chlorophenol:oxygen 1,6-oxidoreductase (decyclizing)

Class

Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen

Sysname

2-amino-5-chlorophenol:oxygen 1,6-oxidoreductase (ring-opening)

Reaction(IUBMB)

2-amino-5-chlorophenol + O2 = 2-amino-5-chloromuconate 6-semialdehyde [RN:R10521]

Reaction(KEGG)

R10521

Substrate

2-amino-5-chlorophenol [CPD:C20669];
O2 [CPD:C00007]

Product

2-amino-5-chloromuconate 6-semialdehyde [CPD:C20670]

Comment

The enzyme, a member of the nonheme-iron(II)-dependent dioxygenase family, is an extradiol-type dioxygenase that utilizes a non-heme ferrous iron to cleave the aromatic ring at the meta position (relative to the hydroxyl substituent). The enzyme from the bacterium Comamonas testosteroni CNB-1 also has the activity of EC 1.13.11.74, 2-aminophenol 1,6-dioxygenase.

History

EC 1.13.11.76 created 2013

Orthology

K15059

2-aminophenol/2-amino-5-chlorophenol 1,6-dioxygenase subunit beta

Genes

PPW:	PputW619_2014

PKC:	PKB_3263(amnb1) PKB_3611(amnb3)

BXE:	Bxe_A1145(amnB)

BXB:	DR64_3304(amnB)

BAV:	BAV0550(amnB)

BHZ:	ACR54_00822(cnbCb)

AXY:	AXYL_02641

AIS:	BUW96_03280

ASW:	CVS48_11345

ACHB:

DVB37_23465

ADY:	HLG70_04300(cnbCb)

APES:

FOC84_32620

AAEG:

RA224_19710(cnbCb)

PIG:	EGT29_21585

NARM:

N7E01_09075(cnbCb)

NHUM:

PQ457_19240 PQ457_19280

SYB:	TZ53_02830

SUFL:

FIL70_13995

SBAR:

H5V43_15190

GSO:	PH603_12425

ARHD:

VSH64_19025

ACTY:

OG774_07860

VMA:	VAB18032_17825

MCRA:

ID554_30935

VER:	HUT12_13600

PRY:	Prubr_10680

SCOR:

J3U87_09070

» show all

Reference

1 [PMID:15580337]

Authors

Wu JF, Sun CW, Jiang CY, Liu ZP, Liu SJ

Title

A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli.

Journal

Arch Microbiol 183:1-8 (2005)
DOI:10.1007/s00203-004-0738-5

Sequence

[ag:AAT35226]

Other DBs

ExplorEnz - The Enzyme Database:

1.13.11.76

IUBMB Enzyme Nomenclature:

1.13.11.76

ExPASy - ENZYME nomenclature database:

1.13.11.76

BRENDA, the Enzyme Database:

1.13.11.76

DBGET integrated database retrieval system