KEGG ENZYME: 1.13.11.85

ENZYME: 1.13.11.85

Entry

EC 1.13.11.85               Enzyme

Name

exo-cleaving rubber dioxygenase;
roxA (gene name);
heme-dependent rubber oxygenase (ambiguous)

Class

Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen

Sysname

cis-1,4-polyisoprene:oxygen dioxygenase [(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal-forming]

Reaction(IUBMB)

cis-1,4-polyisoprene + n O2 = n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal [RN:R11999]

Reaction(KEGG)

R11999

Substrate

cis-1,4-polyisoprene [CPD:C02321];
O2 [CPD:C00007]

Product

(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal [CPD:C21834]

Comment

The enzyme, studied mainly from the bacterium Xanthomonas sp. 35Y, catalyses the cleavage of the double bonds in natural and synthetic rubber (cis-1,4-polyisoprene polymers), generating ends that contain ketone and aldehyde groups. The enzyme from Xanthomonas sp. 35Y contains two c-type cytochromes. It attacks the substrate from its end, producing a single product of 15 carbons.

History

EC 1.13.11.85 created 2018

Orthology

K22690

exo-cleaving rubber dioxygenase

Genes

KAK:	Kalk_04905

MPAF:

R5R33_16320

MBRG:

PVT68_03885

SOK:	D0B54_19000

FCE:	JN531_009745

METP:

C1M51_13405

RGU:	A4W93_07150

MYM:	A176_007569

MFB:	MFUL124B02_27385

CCX:	COCOR_02166

MFU:	LILAB_11505

MMAS:

MYMAC_006684

AVM:	JQX13_05245

MRM:	A7982_11642

HOH:

Hoch_1661

NANN:

O0S08_03710

» show all

Reference

1 [PMID:16348100]

Authors

Tsuchii A, Takeda K

Title

Rubber-degrading enzyme from a bacterial culture.

Journal

Appl Environ Microbiol 56:269-74 (1990)
DOI:10.1128/AEM.56.1.269-274.1990

Reference

2 [PMID:12855168]

Authors

Jendrossek D, Reinhardt S

Title

Sequence analysis of a gene product synthesized by Xanthomonas sp. during growth on natural rubber latex.

Journal

FEMS Microbiol Lett 224:61-5 (2003)
DOI:10.1016/S0378-1097(03)00424-5

Reference

3 [PMID:15574940]

Authors

Braaz R, Fischer P, Jendrossek D

Title

Novel type of heme-dependent oxygenase catalyzes oxidative cleavage of rubber (poly-cis-1,4-isoprene).

Journal

Appl Environ Microbiol 70:7388-95 (2004)
DOI:10.1128/AEM.70.12.7388-7395.2004

Reference

4 [PMID:15870336]

Authors

Braaz R, Armbruster W, Jendrossek D

Title

Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-Isoprene) by a dioxygenase mechanism.

Journal

Appl Environ Microbiol 71:2473-8 (2005)
DOI:10.1128/AEM.71.5.2473-2478.2005

Sequence

[ag:AGT20506]

Reference

5 [PMID:23922395]

Authors

Seidel J, Schmitt G, Hoffmann M, Jendrossek D, Einsle O

Title

Structure of the processive rubber oxygenase RoxA from Xanthomonas sp.

Journal

Proc Natl Acad Sci U S A 110:13833-8 (2013)
DOI:10.1073/pnas.1305560110

Sequence

[ag:AGT20506]

Reference

6 [PMID:24907333]

Authors

Birke J, Jendrossek D

Title

Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms.

Journal

Appl Environ Microbiol 80:5012-20 (2014)
DOI:10.1128/AEM.01271-14

Other DBs

ExplorEnz - The Enzyme Database:

1.13.11.85

IUBMB Enzyme Nomenclature:

1.13.11.85

ExPASy - ENZYME nomenclature database:

1.13.11.85

BRENDA, the Enzyme Database:

1.13.11.85

All links

Chemical substance (3)   
   KEGG COMPOUND (3)   
Chemical reaction (1)   
   KEGG REACTION (1)   
Gene (21)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (17)   
   KEGG MGENES (3)   
All databases (25)   

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