KEGG   ENZYME: 1.13.11.87
Entry
EC 1.13.11.87               Enzyme                                 
Name
endo-cleaving rubber dioxygenase;
latex clearing protein;
lcp (gene name);
roxB (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
cis-1,4-polyisoprene:oxygen dioxygenase (endo-cleaving)
Reaction(IUBMB)
Cleavage of cis-1,4-polyisoprene polymers into a mixture of compounds, including a C20 compound ((4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal), a C25 compound ((4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal), a C30 compound ((4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal), and larger isoprenologes such as C35, C40, C45, and higher analogues.
Comment
The enzyme catalyses the cleavage of the double bonds in natural and synthetic rubber, producing a mixture of C20, C25, C30, and higher oligo-isoprenoids with ketone and aldehyde groups at their ends. Two unrelated bacterial enzymes are known to possess this activity - the enzyme from Streptomyces sp. K30 (Lcp) contains a b-type cytochrome, while the enzyme from Xanthomonas sp. 35Y, (RoxB) contains two c-type cytochromes. Both enzymes attack the substrate at random locations, and are not able to cleave the C35 or smaller products into shorter fragments.
History
EC 1.13.11.87 created 2018
Orthology
K23117  endo-cleaving rubber dioxygenase
K23118  endo-cleaving rubber dioxygenase
Genes
KAKKalk_09255
SNANI6N98_07925 I6N98_07935 I6N98_16095 I6N98_16100 I6N98_16105 I6N98_16130 I6N98_16165 I6N98_16170 I6N98_16195
MPAFR5R33_08940
MBRGPVT68_10440
SOKD0B54_06115 D0B54_06125 D0B54_14040 D0B54_15470 D0B54_18995 D0B54_19015 D0B54_23695
SINIGT972_12125 GT972_12265
RGUA4W93_01825
MYMA176_005221 A176_005491
MFBMFUL124B02_11685 MFUL124B02_36925
CCXCOCOR_02837
MRMA7982_05515
HOHHoch_1446
NANNO0S08_00340
ASDAS9A_0019
NFANFA_21740
NFRERS450000_01925(lcp_2)
NCYNOCYR_2698
NBRO3I_014870
NNONONO_c40940(lcp)
NSLBOX37_24960
NOZDMB37_35475
NODFOH10_10075
NADNCTC11293_02479(lcp_1)
NWLNWFMUON74_36450
NIEKV110_18845
NTCKHQ06_19760
NHUH0264_21565
NYALTV02_08325
NGPLTT66_26785
NSPUIFM12276_41480
NYUD7D52_27315
RBYCEJ39_06875
RRT4535765_00883(lcp_1)
RCRNCTC10994_01110(lcp_1)
RTMG4H71_16485
RGOKYT97_18265
GPOGPOL_174p00150(lcp2) GPOL_c48310(lcp1)
GORKTR9_1229
GTABCM27_07160
GITC6V83_05605
GRUGCWB2_06690(lcp2)
GOMD7316_00417(lcp_1)
GAVC5O27_21075
GODGKZ92_06265
GAMGII34_21685
GPDGII33_21385
GJIH1R19_06460
GHNMVF96_06590
GAMIIHQ52_09435
GMGNWF22_11910
GSIP5P27_03620
TSMASU32_02530
TPULTPB0596_04930
SCOSCO6780(SC6A5.29)
SSXSACTE_6144
SFASfla_5664
STRPF750_0385 F750_0935
SLVSLIV_04620(lcp)
SCZABE83_07250 ABE83_07945 ABE83_07950
STRFASR50_31060
SLEsle_64270(sle_64270) sle_64280(sle_64280)
SPHWNFX46_16955
SVUB1H20_29770
SGDELQ87_03865
SNKCP967_01650 CP967_04590
SFUGCNQ36_01190 CNQ36_03940 CNQ36_03945
SPRACP972_05110 CP972_26845 CP972_26850
SXNIAG42_02900
SGJIAG43_29120 IAG43_29125
SCIRSTRCI_000262 STRCI_000263 STRCI_007870
SLONLGI35_37055
AARCG127AT_10675
AMAVGCM10025877_29750
ALAVMTO99_02670
ARIUM93_14895
NOIFCL41_07575
MUZH4N58_07330 H4N58_14215
TCUTcur_0105
ACTWF7P10_24280
AMAZLUW76_24185
ACIROG979_11795 OG979_36420
SROSros_5156
NOABKM31_16705
NCXNocox_19325(lcp1) Nocox_19330(lcp2) Nocox_38035(lcp4)
NGNLCN96_27045 LCN96_27050
NFSOIE67_31390 OIE67_31395
AMDAMED_3299
AMNRAM_16785
AMMAMES_3263
AMZB737_3263
AJAAJAP_12695(lcp1) AJAP_12715(lcp2)
AMYCCU254_04705
AMYBBKN51_16695
AABA4R43_01335 A4R43_01355
AMYYYIM_18850(lcp1) YIM_34625(lcp2)
AORISD37_24020
APRTMUY14_19500 MUY14_19940
AROONQK81_40065
ATHML1857_18445 L1857_18455 L1857_18850
APRECNX65_23345
AMIAmir_4374 Amir_4375 Amir_4686
KALKALB_2392
KUTJJ691_20350(lcp)
KPHYAOZ06_17885 AOZ06_17890
LEDBBK82_34125 BBK82_34130
PMADBAY61_05255 BAY61_05260
MAUMicau_2134 Micau_2138
MILML5_2239 ML5_2243
MICBMicB006_5198 MicB006_5202
MTUACSH63_14090 CSH63_14110
MPRNQ3V37_19220 Q3V37_19250
AMSAMIS_36550 AMIS_45690 AMIS_45700
ACTLL3i22_063830
ASICQ0Z83_014670 Q0Z83_020180 Q0Z83_020190
PRYPrubr_74320
MICAP0L94_07335 P0L94_15340
 » show all
Reference
1  [PMID:16348100]
  Authors
Tsuchii A, Takeda K
  Title
Rubber-degrading enzyme from a bacterial culture.
  Journal
Appl Environ Microbiol 56:269-74 (1990)
DOI:10.1128/AEM.56.1.269-274.1990
Reference
2  [PMID:12855168]
  Authors
Jendrossek D, Reinhardt S
  Title
Sequence analysis of a gene product synthesized by Xanthomonas sp. during growth  on natural rubber latex.
  Journal
FEMS Microbiol Lett 224:61-5 (2003)
DOI:10.1016/S0378-1097(03)00424-5
Reference
3  [PMID:15574940]
  Authors
Braaz R, Fischer P, Jendrossek D
  Title
Novel type of heme-dependent oxygenase catalyzes oxidative cleavage of rubber (poly-cis-1,4-isoprene).
  Journal
Appl Environ Microbiol 70:7388-95 (2004)
DOI:10.1128/AEM.70.12.7388-7395.2004
Reference
4  [PMID:15870336]
  Authors
Braaz R, Armbruster W, Jendrossek D
  Title
Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-Isoprene) by a dioxygenase mechanism.
  Journal
Appl Environ Microbiol 71:2473-8 (2005)
DOI:10.1128/AEM.71.5.2473-2478.2005
Reference
5  [PMID:23922395]
  Authors
Seidel J, Schmitt G, Hoffmann M, Jendrossek D, Einsle O
  Title
Structure of the processive rubber oxygenase RoxA from Xanthomonas sp.
  Journal
Proc Natl Acad Sci U S A 110:13833-8 (2013)
DOI:10.1073/pnas.1305560110
Reference
6  [PMID:24907333]
  Authors
Birke J, Jendrossek D
  Title
Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms.
  Journal
Appl Environ Microbiol 80:5012-20 (2014)
DOI:10.1128/AEM.01271-14
  Sequence
Reference
7  [PMID:28500046]
  Authors
Birke J, Rother W, Jendrossek D
  Title
RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp).
  Journal
Appl Environ Microbiol 83:e00721-17 (2017)
DOI:10.1128/AEM.00721-17
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.87
IUBMB Enzyme Nomenclature: 1.13.11.87
ExPASy - ENZYME nomenclature database: 1.13.11.87
BRENDA, the Enzyme Database: 1.13.11.87

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