KEGG ENZYME: 1.14.11.20

ENZYME: 1.14.11.20

Entry

EC 1.14.11.20               Enzyme

Name

deacetoxyvindoline 4-hydroxylase;
desacetoxyvindoline 4-hydroxylase;
desacetyoxyvindoline-17-hydroxylase;
D17H;
desacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Sysname

deacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)

Reaction(IUBMB)

deacetoxyvindoline + 2-oxoglutarate + O2 = deacetylvindoline + succinate + CO2 [RN:R05857]

Reaction(KEGG)

R05857

Substrate

deacetoxyvindoline [CPD:C02673];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

Product

deacetylvindoline [CPD:C01091];
succinate [CPD:C00042];
CO2 [CPD:C00011]

Comment

Requires Fe2+ and ascorbate. Also acts on 3-hydroxy-16-methoxy-2,3-dihydrotabersonine and to a lesser extent on 16-methoxy-2,3-dihydrotabersonine.

History

EC 1.14.11.20 created 2002, modified 2005

Pathway

ec00901

Indole alkaloid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12697

deacetoxyvindoline 4-hydroxylase

Reference

1 [PMID:16667836]

Authors

De Carolis E, Chan F, Balsevich J, De Luca V

Title

Isolation and Characterization of a 2-Oxoglutarate Dependent Dioxygenase Involved in the Second-to-Last Step in Vindoline Biosynthesis.

Journal

Plant Physiol 94:1323-1329 (1990)
DOI:10.1104/pp.94.3.1323

Sequence

[ag:AAB97311]

Reference

2 [PMID:8449913]

Authors

De Carolis E, De Luca V

Title

Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus.

Journal

J Biol Chem 268:5504-11 (1993)

Sequence

[ag:AAB97311]

Reference

3 [PMID:9701591]

Authors

Vazquez-Flota FA, De Luca V.

Title

Developmental and light regulation of desacetoxyvindoline 4-hydroxylase in catharanthus roseus (L.) G. Don. . Evidence Of a multilevel regulatory mechanism

Journal

Plant Physiol 117:1351-61 (1998)
DOI:10.1104/pp.117.4.1351

Other DBs

ExplorEnz - The Enzyme Database:

1.14.11.20

IUBMB Enzyme Nomenclature:

1.14.11.20

ExPASy - ENZYME nomenclature database:

1.14.11.20

BRENDA, the Enzyme Database:

1.14.11.20

CAS:	132084-83-4

DBGET integrated database retrieval system