KEGG ENZYME: 1.14.11.36

ENZYME: 1.14.11.36

Entry

EC 1.14.11.36               Enzyme

Name

pentalenolactone F synthase;
penD (gene name);
pntD (gene name);
ptlD (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Sysname

pentalenolactone-D,2-oxoglutarate:oxygen oxidoreductase

Reaction(IUBMB)

pentalenolactone D + 2 2-oxoglutarate + 2 O2 = pentalenolactone F + 2 succinate + 2 CO2 + H2O (overall reaction) [RN:R10145];
(1a) pentalenolactone D + 2-oxoglutarate + O2 = pentalenolactone E + succinate + CO2 + H2O [RN:R10143];
(1b) pentalenolactone E + 2-oxoglutarate + O2 = pentalenolactone F + succinate + CO2 [RN:R10144]

Reaction(KEGG)

R10143 R10144 R10145

Substrate

pentalenolactone D [CPD:C20399];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007];
pentalenolactone E [CPD:C20398]

Product

pentalenolactone F [CPD:C20397];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001];
pentalenolactone E [CPD:C20398]

Comment

Requires iron(II) and ascorbate. Isolated from the bacteria Streptomyces exfoliatus, Streptomyces arenae and Streptomyces avermitilis. Part of the pentalenolactone biosynthesis pathway.

History

EC 1.14.11.36 created 2012

Pathway

ec00998

Biosynthesis of various antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K18057

pentalenolactone F synthase

Genes

SMA:	SAVERM_2995(ptlD)

SBH:

SBI_09677

SCI:	B446_29685

SCW:	TU94_27395

SLE:	sle_13180(sle_13180)

SSIA:

A7J05_09250

SGJ:	IAG43_27145

SHK:	J2N69_35815

Reference

1 [PMID:21250661]

Authors

Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE

Title

Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone.

Journal

Biochemistry 50:1739-54 (2011)
DOI:10.1021/bi1019786

Sequence

[sma:SAVERM_2995]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.11.36

IUBMB Enzyme Nomenclature:

1.14.11.36

ExPASy - ENZYME nomenclature database:

1.14.11.36

BRENDA, the Enzyme Database:

1.14.11.36

DBGET integrated database retrieval system