KEGG ENZYME: 1.14.11.38

ENZYME: 1.14.11.38

Entry

EC 1.14.11.38               Enzyme

Name

verruculogen synthase;
fmtF (gene name);
FmtOx1

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Sysname

fumitremorgin B,2-oxoglutarate:oxygen oxidoreductase (verruculogen-forming)

Reaction(IUBMB)

fumitremorgin B + 2-oxoglutarate + 2 O2 + reduced acceptor = verruculogen + succinate + CO2 + H2O + acceptor [RN:R10445]

Reaction(KEGG)

R10445

Substrate

fumitremorgin B [CPD:C20630];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007];
reduced acceptor [CPD:C00030]

Product

verruculogen [CPD:C20045];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001];
acceptor [CPD:C00028]

Comment

Requires Fe2+ and ascorbate. Found in the fungus Aspergillus fumigatus. Both atoms of a dioxygen molecule are incorporated into verruculogen [1,2]. Involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgin A.

History

EC 1.14.11.38 created 2013

Pathway

ec00404

Staurosporine biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K17825

verruculogen synthase

Genes

MNZ:

135195090

AFM:	AFUA_8G00230

NFI:	NFIA_045530 NFIA_093740

URE:	UREG_04217 UREG_07661

Reference

1 [PMID:19763315]

Authors

Steffan N, Grundmann A, Afiyatullov S, Ruan H, Li SM

Title

FtmOx1, a non-heme Fe(II) and alpha-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus.

Journal

Org Biomol Chem 7:4082-7 (2009)
DOI:10.1039/b908392h

Sequence

[afm:AFUA_8G00230]

Reference

2 [PMID:21404415]

Authors

Kato N, Suzuki H, Takagi H, Uramoto M, Takahashi S, Osada H

Title

Gene disruption and biochemical characterization of verruculogen synthase of Aspergillus fumigatus.

Journal

Chembiochem 12:711-4 (2011)
DOI:10.1002/cbic.201000562