KEGG ENZYME: 1.14.11.39

ENZYME: 1.14.11.39

Entry

EC 1.14.11.39               Enzyme

Name

L-asparagine hydroxylase;
L-asparagine 3-hydroxylase;
AsnO

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Sysname

L-asparagine,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)

Reaction(IUBMB)

L-asparagine + 2-oxoglutarate + O2 = (2S,3S)-3-hydroxyasparagine + succinate + CO2 [RN:R10446]

Reaction(KEGG)

R10446

Substrate

L-asparagine [CPD:C00152];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

Product

(2S,3S)-3-hydroxyasparagine [CPD:C20631];
succinate [CPD:C00042];
CO2 [CPD:C00011]

Comment

Requires Fe2+. The enzyme is only able to hydroxylate free L-asparagine. It is not active toward D-asparagine. The beta-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone.

History

EC 1.14.11.39 created 2013

Orthology

K18058

L-asparagine oxygenase

Genes

ENC:

ECL_03968

ECLG:

EC036_33910

EMOR:

L6Y89_16585

SMAC:

SMDB11_0605 SMDB11_1104

SRR:	SerAS9_0274

SPLY:

Q5A_006790(asnO)

SRS:	SerAS12_0274

SRA:	SerAS13_0273

SFG:	AV650_20270

SSUR:

ATE40_003415

SNEV:

OI978_27090

RAQ:	Rahaq2_2682

ROX:	BV494_24950

SERA:

Ser39006_015050

SERQ:

CWC46_15045

DSO:	A4U42_21025

CED:	LH89_00095 LH89_22315

DFN:	CVE23_12695

DAQ:	DAQ1742_04254

DLC:	O1Q98_07925

DDA:	Dd703_1456

ETA:

ETA_15700

PALF:

K6R05_18895

PLU:	plu3528 plu3801

PLUM:

A4R40_17540 A4R40_18915

PTT:	VY86_04825

XBV:	XBW1_mp0174

XNE:	XNC1_2155 XNC1_2460 XNC1_4045

XNM:	XNC2_1351 XNC2_2084 XNC2_2366 XNC2_3898

XPO:	XPG1_2874 XPG1_3240

XHO:	A9255_19685

PTHA:

OI982_14310

HIF:	HIBPF_07710

HIL:	HICON_17210

HAEG:

NCTC8502_01634(asnO)

XFM:	Xfasm12_0852

XFL:	P303_03580 P303_04050

FAU:	Fraau_1426

VTU:	IX91_10335

VAQ:	FIV01_12890(mppO)

VSR:	Vspart_00116(asnO_1)

VSA:	VSAL_I1305

PMAI:

CF386_12490

PPG:	PputGB1_5419

PPW:	PputW619_0540

PPUT:

L483_02555

PPUN:

PP4_05200

PSP:	PSPPH_4996

PSYI:

MME58_00600

PFE:	PSF113_0453b

PFB:

VO64_2148

PTOL:

I7845_15670

PSV:	PVLB_22765

PPV:	NJ69_17710

PSOS:

POS17_3974(PputW619_0540)

PPSL:

BJP27_20500

PANH:

HU763_002420

PORY:

EJA05_00025

PSIH:

LOY51_00025

PKM:	PZ739_02550

PNB:	NK667_23425

PPAE:

LDL65_21495

PSA:

PST_3923

PSZ:	PSTAB_3884(mppO)

PSR:	PSTAA_4073

PSC:	A458_01685

PSJ:	PSJM300_01810

PSH:	Psest_0326

PSTU:

UIB01_01625

PSED:

DM292_20880

PCHL:

LLJ08_01665

PKG:	LW136_01665

PZD:	KQ248_06755

ASHA:

G8E00_11445

MOI:	MOVS_02950

MCUN:

NCTC10297_00742

SHP:	Sput200_2525

PRR:	AT705_10755 AT705_23320

PMAZ:

R5H13_10015

CEB:	B0D95_16160

LLO:

LLO_3359

MPSY:

CEK71_21840

HBH:	E4T21_00675

BTHG:

MS2017_1703

NCI:	NCTC10296_01180(asnO)

BCM:	Bcenmc03_6386

BCH:	Bcen2424_5877

BCJ:

BCAS0290

BCEO:

I35_7306

BCED:

DM42_6785

BCEP:

APZ15_35315

BPYR:

ABD05_30850 ABD05_32945

BSEM:

WJ12_20955

BMEC:

WJ16_18780

BSTL:

BBJ41_33110

BARI:

NLX30_36345

BURK:

DM992_33855

BCAI:

K788_0005646

PBRY:

NDK50_01175

HYF:	DTO96_101676

BBRO:

BAU06_22245

BFZ:	BAU07_22400

BOH:	AKI39_03465

BGV:	CAL12_24355

KIA:	G8A07_15085

JAL:	BZG29_19805

HHT:	F506_00300

THI:

THI_1730

FKU:	FGKAn22_03770

RBE:

RBE_0478

RBO:

A1I_02770

AMIH:

CO731_01849

BIC:	LMTR13_25260

KVL:	KVU_PA0021

KVU:

EIO_3104

CFUS:

CYFUS_003163

BSIA:

CWD84_18685

BWH:	A9C19_04265

BACS:

AUL54_07450

PARH:

I5S86_03595

PFRI:

L8956_04705

BHUI:

LOK74_08285

KEB:	GXN75_09265

PABS:

JIR001_15040(asnO_1)

MSA:	Mycsm_07253

CPU:	CPFRC_03130

CPL:	Cp3995_0633(tauD)

CPG:	CP316_03250

CPP:	CpP54B96_0633(tauD)

CPK:	CP1002_10005

CPQ:	CPC231_03130

CPX:	CPI19_08390

CPZ:	CpPAT10_0623(tauD)

COR:	Cp267_0650(tauD)

COP:	CP31_03485

COD:	Cp106_0606(tauD)

COS:	Cp4202_0616(tauD)

COI:	CPCIP5297_03275

COE:	CP258_03270

COU:	CP162_03155

CPSE:

CPTA_01173

CPSU:

CPTB_00359

CPSF:

CPTC_01163

CUL:	CULC22_00672(ogs)

CUC:	CULC809_00665(ogs)

CUE:	CULC0102_0775(ogs)

CUN:	Cul210932_0700(tauD)

CUQ:	Cul210931_0669(tauD)

CUZ:	Cul05146_0718(tauD)

CUJ:	CUL131002_0678c(tauD)

CUS:	CulFRC11_0669(tauD)

CGY:	CGLY_00545 CGLY_11835

CSIL:

CBE74_03910

NFA:

NFA_48610

NFR:	ERS450000_05049(asnO)

NCY:	NOCYR_4655

NBR:	O3I_036890

NNO:	NONO_c66170

NSL:	BOX37_26935

NSR:	NS506_01690(asnO)

NTP:	CRH09_30780 CRH09_34590

NOZ:	DMB37_15820 DMB37_36755

NOD:	FOH10_01930

NAH:	F5544_17945 F5544_17970 F5544_39350

NAD:	NCTC11293_06239(asnO_2)

NWL:	NWFMUON74_10480

NIE:	KV110_36135

ROA:	Pd630_LPD16019

ROZ:	CBI38_01540

GOM:	D7316_02856(mppO)

SRT:

Srot_0774

SCO:	SCO2693(SCC61A.14) SCO3236(SCE29.05c)

SALB:

XNR_0986

SGB:

WQO_12245

SFI:

SFUL_5831

SGRF:

SGFS_011610 SGFS_025000 SGFS_103200

SCYE:

R2B67_06775

SCB:	SCAB_3341 SCAB_5271

SSX:	SACTE_6543

SVL:	Strvi_3124

SHY:

SHJG_1397

SHO:	SHJGH_1231

SRC:	M271_40120

SBH:	SBI_00878 SBI_00941 SBI_09256

SDV:	BN159_6047

SALS:

SLNWT_6835

STRP:

F750_6418

SCI:	B446_23870

SLV:	SLIV_21510(asnO1) SLIV_24200(asnO2)

SGU:	SGLAU_07120

SVT:	SVTN_22095

STRE:

GZL_01725

SLD:	T261_2127 T261_7562

SPRI:

SPRI_3466

SCZ:	ABE83_06835 ABE83_06955

SCX:	AS200_25135

SRW:	TUE45_06291(mppO_1) TUE45_06409(asnO_5) TUE45_06920(mppO_2) TUE45_pSRc_0447(vioC)

SRN:	A4G23_02365(asnO_1) A4G23_04203 A4G23_04388(vioC) A4G23_05516

SCLF:

BB341_15030

STSI:

A4E84_04350

SKY:	D0C37_06320 D0C37_30510

SALW:

CP975_03210 CP975_28170

SLS:	SLINC_4870

SALU:

DC74_5997

SALL:

SAZ_31060

SPLU:

LK06_021015

SNW:	BBN63_06790 BBN63_30085

SSIA:

A7J05_00255 A7J05_03135 A7J05_07200 A7J05_36480

SVU:	B1H20_11480

SGV:	B1H19_08945 B1H19_09120 B1H19_35575

SLAU:

SLA_5783

SALJ:

SMD11_0981 SMD11_3232

SLX:	SLAV_05480(mppO) SLAV_07260(asnO)

SFK:

KY5_0486c

SGE:	DWG14_08222(mppO)

SLK:	SLUN_13775 SLUN_22945

SDX:	C4B68_01260 C4B68_36880

SGD:	ELQ87_17590

SAST:

CD934_30580

SNQ:	CP978_01995

STIR:

DDW44_23825

SKA:	CP970_40345

SGZ:	C0216_32755(vioC)

SVN:	CP980_28965

SNK:	CP967_17245

SRK:	FGW37_28385

SAQU:

EJC51_46700

SCAV:

CVT27_26855

SSEO:

D0Z67_22420

SBY:	H7H31_00850

SRIM:

CP984_39215

SPHV:

F9278_45090

SCIN:

CP977_31230

SCHA:

CP983_18395

SGX:	H4W23_33125

SBAT:

G4Z16_04870

SNF:	JYK04_08164(asnO_2)

SPLA:

CP981_21715

SATA:

C5746_39315

SHUN:

DWB77_00456(asnO_1)

SLIA:

HA039_20700

SDW:	K7C20_27875

SCAL:

I6J39_11685 I6J39_22910

SFB:	CP974_12965 CP974_22660 CP974_29360

SROI:

IAG44_03950

SXN:	IAG42_37060 IAG42_37075

SHK:	J2N69_28135

STRY:

EQG64_28155

SDEC:

L3078_05850

SDUR:

M4V62_11920

SAKB:

K1J60_40335

STAA:

LDH80_07070

SCAE:

IHE65_39930

SXT:	KPP03845_101305(asnO_1) KPP03845_101317(asnO_2)

SLON:

LGI35_02110

SANT:

QR300_08105

SOV:	QZH56_21220

SFIY:

F0344_15560

SCOA:

QU709_17575

SROC:

RGF97_00815

SCHG:

NRO40_26770

SINN:

ABB07_33855

SCT:	SCAT_2689(asnO) SCAT_p1377

SCY:	SCATT_26730 SCATT_p03480

KAB:	B7C62_11150 B7C62_26740

KIT:	CFP65_1227

STRH:

GXP74_29695

MSF:	IT882_06300

RTN:	A6122_0640

RRY:	C1O28_01960

RTE:	GSU10_09185

PNV:	JMY29_00220

NCA:

Noca_1042

KQI:	F1D05_30950

SRO:

Sros_3755

NOA:	BKM31_45775

FAL:

FRAAL4688

KRA:

Krad_3958

SEN:	SACE_4278 SACE_5782

AOI:

AORI_3167

AJA:	AJAP_23525

AMYC:

CU254_26135

AMYY:

YIM_25785(asnO3)

AORI:

SD37_09295 SD37_16155

AROO:

NQK81_06740

APRE:

CNX65_18320

SSYI:

EKG83_30670

KAL:	KALB_1756 KALB_2593 KALB_5896 KALB_6160

KUT:	JJ691_22670

LED:	BBK82_35715

ACTA:

C1701_17135 C1701_25725

ALO:

CRK62029

ACTU:

Actkin_01205(asnO_1) Actkin_01360(asnO_2)

CROS:

N8J89_15355

MAU:	Micau_1827

MTEM:

GCE86_18175

VMA:	VAB18032_03125

MCRA:

ID554_06655 ID554_16940

VER:	HUT12_06305 HUT12_30735

MCHL:

PVK74_09680

MPRN:

Q3V37_03705

MHAW:

RMN56_19205

ASE:	ACPL_2187(cs1)

ACTN:

L083_4621

ACTS:

ACWT_2065

AOU:	ACTOB_006425

PLK:	CIK06_09170(vioC)

PLAB:

C6361_01855 C6361_01950

PLAT:

C6W10_19720 C6W10_19815

PSUU:

Psuf_065540(asnO)

CAI:	Caci_0231 Caci_6328

RXY:

Rxyl_1155

CYB:

CYB_1854

LET:	O77CONTIG1_00553(cs2)

THEU:

HPC62_14935

TOG:	HNI00_17820

TSIN:

OXH18_00750

GEN:	GM3709_3716

KBB:	ccbrp13_70410

GES:	VT84_06800(asnO)

CPI:

Cpin_2834

CHIH:

GWR21_13095

NKO:	Niako_2766

FLN:

FLA_1331

FUA:	LVD17_26735

FJO:

Fjoh_3169

CIO:	CEQ15_06495

CHRY:

CEY12_03435

CJT:	EG359_03155

CCAU:

EG346_19170

CGLE:

NCTC11432_00670(asnO)

» show all

Reference

1 [PMID:17373765]

Authors

Strieker M, Kopp F, Mahlert C, Essen LO, Marahiel MA

Title

Mechanistic and structural basis of stereospecific Cbeta-hydroxylation in calcium-dependent antibiotic, a daptomycin-type lipopeptide.

Journal

ACS Chem Biol 2:187-96 (2007)
DOI:10.1021/cb700012y

Sequence

[sco:SCO3236]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.11.39

IUBMB Enzyme Nomenclature:

1.14.11.39

ExPASy - ENZYME nomenclature database:

1.14.11.39

BRENDA, the Enzyme Database:

1.14.11.39

DBGET integrated database retrieval system