KEGG ENZYME: 1.14.11.52

ENZYME: 1.14.11.52

Entry

EC 1.14.11.52               Enzyme

Name

validamycin A dioxygenase;
vldW (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Sysname

validamycin-A,2-oxoglutarate:oxygen oxidoreductase (6'-hydroxylating)

Reaction(IUBMB)

validamycin A + 2-oxoglutarate + O2 = validamycin B + succinate + CO2 [RN:R11340]

Reaction(KEGG)

R11340

Substrate

validamycin A [CPD:C12112];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

Product

validamycin B [CPD:C21293];
succinate [CPD:C00042];
CO2 [CPD:C00011]

Comment

The enzyme was characterized from the bacterium Streptomyces hygroscopicus subsp. limoneus. Requires Fe2+.

History

EC 1.14.11.52 created 2016

Pathway

ec00525

Acarbose and validamycin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K21036

validamycin A dioxygenase

Genes

SHY:	SHJG_0280 SHJG_0285

SHO:	SHJGH_0114 SHJGH_0119

SINE:

KI385_04775

STRH:

GXP74_22190

Reference

1 [PMID:22961651]

Authors

Almabruk KH, Asamizu S, Chang A, Varghese SG, Mahmud T

Title

The alpha-ketoglutarate/Fe(II)-dependent dioxygenase VldW is responsible for the formation of validamycin B.

Journal

Chembiochem 13:2209-11 (2012)
DOI:10.1002/cbic.201200464

Other DBs

ExplorEnz - The Enzyme Database:

1.14.11.52

IUBMB Enzyme Nomenclature:

1.14.11.52

ExPASy - ENZYME nomenclature database:

1.14.11.52

BRENDA, the Enzyme Database:

1.14.11.52

DBGET integrated database retrieval system