KEGG ENZYME: 1.14.11.6

ENZYME: 1.14.11.6

Entry

EC 1.14.11.6                Enzyme

Name

thymine dioxygenase;
thymine 7-hydroxylase;
5-hydroxy-methyluracil dioxygenase;
5-hydroxymethyluracil oxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Sysname

thymine,2-oxoglutarate:oxygen oxidoreductase (7-hydroxylating)

Reaction(IUBMB)

thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2 [RN:R01412]

Reaction(KEGG)

R01412;
(other) R08610 R08611

Substrate

thymine [CPD:C00178];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

Product

5-hydroxymethyluracil [CPD:C03088];
succinate [CPD:C00042];
CO2 [CPD:C00011]

Comment

Requires Fe2+ and ascorbate. Also acts on 5-hydroxymethyluracil to oxidize its -CH2OH group first to -CHO and then to -COOH.

History

EC 1.14.11.6 created 1972, modified 1976 (EC 1.14.11.5 created 1972, incorporated 1976)

Orthology

K22406

thymine dioxygenase

K22407

thymine dioxygenase / DNA helicase

Genes

TBR:	Tb11.01.5220 Tb927.7.4650

TBG:	TbgDal_VII5260 TbgDal_XI15230

TCR:	508859.74 510357.10

LMA:	LMJF_09_1480(JBP1) LMJF_14_0040

LIF:	LINJ_09_1560(JBP1) LINJ_14_0040

LDO:	LDBPK_091560 LDBPK_140040

LMI:	LMXM_09_1480 LMXM_14_0040

LBZ:	LBRM_09_1540 LBRM_14_0040

LPAN:

LPMP_091480(JBP1) LPMP_140040

Reference

1 [PMID:11946494]

Authors

Bankel L, Holme E, Lindstedt G, Lindstedt S.

Title

Oxygenases involved in thymine and thymidine metabolism in Neurospora crassa.

Journal

FEBS Lett 21:135-138 (1972)
DOI:10.1016/0014-5793(72)80121-2

Reference

2 [PMID:4274083]

Authors

Liu CK, Hsu CA, Abbott MT.

Title

Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase.

Journal

Arch Biochem Biophys 159:180-7 (1973)
DOI:10.1016/0003-9861(73)90443-8

Reference

3 [PMID:6684117]

Authors

Warn-Cramer BJ, Macrander LA, Abbott MT.

Title

Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis.

Journal

J Biol Chem 258:10551-7 (1983)

Other DBs

ExplorEnz - The Enzyme Database:

1.14.11.6

IUBMB Enzyme Nomenclature:

1.14.11.6

ExPASy - ENZYME nomenclature database:

1.14.11.6

BRENDA, the Enzyme Database:

1.14.11.6

CAS:	37256-67-0

DBGET integrated database retrieval system