KEGG   ENZYME: 1.14.11.60
Entry
EC 1.14.11.60               Enzyme                                 
Name
scopoletin 8-hydroxylase;
S8H (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
scopoletin,2-oxoglutarate:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
scopoletin + 2-oxoglutarate + O2 = fraxetin + succinate + CO2 [RN:R12222]
Reaction(KEGG)
R12222
Substrate
scopoletin [CPD:C01752];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
fraxetin [CPD:C09265];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires iron(II) and ascorbate. A protein involved in biosynthesis of iron(III)-chelating coumarins in higher plants.
History
EC 1.14.11.60 created 2018
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01110  Biosynthesis of secondary metabolites
Orthology
K23145  scopoletin 8-hydroxylase
Genes
ATH: AT3G12900
ALY: 9318860
CRB: 17892971
CSAT: 104745510 104748679 104765057 104777120
EUS: EUTSA_v10020841mg
BRP: 103849720 103859475 103870212
BNA: 106345421 106375087 106375542 106382146 106386876 106400351
BOE: 106293989 106301500 106333669
RSZ: 108810911 108835958 108861046
THJ: 104810262
CPAP: 110809857
CIT: 102607640
CIC: CICLE_v10006882mg
PVY: 116119197 116133563
MINC: 123206691 123226450
TCC: 18594550 18594551 18594553 18594556
GRA: 105761379 105775787 105775819 105784310 105784311 105787151 105787154
GHI: 107886197 107911338 107912932 107913465 107919665 107932640 107932661 107932662 107936166 107936167 107936168 107936170 121223450
GAB: 108452509 108461393 108461394 108480494 108480495 108480496 108480497 108482454 108482635
HSYR: 120123825 120127654 120127655 120127656 120129162 120155394 120155446 120161610
DZI: 111276015 111276016 111276018 111276020 111276597 111276599 111276600 111287890 111318382 111318388 111318392
GMX: 100803722 102659647
GSJ: 114423069 114423906
PVU: PHAVU_005G059100g
VRA: 106761811
VAR: 108333781
VUN: 114181474
VUM: 124832952
CCAJ: 109790572 109790573 109790576 109818815
MTR: 11407206 11409249
TPRA: 123898291
CAM: 101492926
PSAT: 127128436
VVO: 131596845 131596847 131598917 131598918 131598919 131598922 131598923 131598924 131598926 131634121 131634122 131634123 131634124 131634125 131634128
LJA: Lj3g3v0965390.1(Lj3g3v0965390.1) Lj3g3v0965390.2(Lj3g3v0965390.2) Lj3g3v0965390.3(Lj3g3v0965390.3)
ADU: 107466129 107471942 107480135
AIP: 107619811 107630421
AHF: 112708410 112711180 112738254 112779478 112791023
LANG: 109333837 109355985
FVE: 105350415
RCN: 112203388
PPER: 18778908
PMUM: 103325695
PAVI: 110768994
PDUL: 117626205
MDM: 103435419 114823354
MSYL: 126623735
PXB: 103952012
MNT: 21399318
CSAV: 115709070
RCU: 107260838 8272615
JCU: 105646195 105646196
HBR: 110638461 110662018 110662019 110662020
MESC: 110617017 110630845 122721679 122723798
POP: 7480442
PEU: 105123997
PALZ: 118054079
JRE: 109007025
CILL: 122311404
CAVE: 132174257
QSU: 111986809 112009979
QLO: 115984049 115984050
TWL: 120009221
VVI: 100259503 100854658 104877301
VRI: 117908689 117908690 117910864
SLY: 101262174
SPEN: 107004094
SOT: 102584589
SSTN: 125844705
SDUL: 129903706 129904688
LBB: 132644063
NTA: 107766774 107821681
NSY: 104226661
NTO: 104118671
NAU: 109238378
INI: 109160293
ITR: 116005047
OEU: 111396786
HAN: 110878125 110878126 110878127 110937900 110939629 110939645 110939654 110939655
ECAD: 122594033
LSV: 111890983 111890990 111891065 111902368
CCAV: 112522925
DCR: 108216893 108218127
CSIN: 114267034
RVL: 131321855
AEW: 130754918 130761620 130773728
NNU: 104603880 104609462
MING: 122059183 122063766 122063776 122067316 122092711
TSS: 122640930 122649187
PSOM: 113289572 113298261 113298269 113326671 113332375
MSIN: 131243293 131243300
ATR: 18424098 18424099 18424100 18424103
 » show all
Reference
1  [PMID:29361149]
  Authors
Siwinska J, Siatkowska K, Olry A, Grosjean J, Hehn A, Bourgaud F, Meharg AA, Carey M, Lojkowska E, Ihnatowicz A
  Title
Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis.
  Journal
J Exp Bot 69:1735-1748 (2018)
DOI:10.1093/jxb/ery005
  Sequence
[ath:AT3G12900]
Reference
2  [PMID:29581584]
  Authors
Rajniak J, Giehl RFH, Chang E, Murgia I, von Wiren N, Sattely ES
  Title
Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
  Journal
Nat Chem Biol 14:442-450 (2018)
DOI:10.1038/s41589-018-0019-2
  Sequence
[ath:AT3G12900]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.60
IUBMB Enzyme Nomenclature: 1.14.11.60
ExPASy - ENZYME nomenclature database: 1.14.11.60
BRENDA, the Enzyme Database: 1.14.11.60

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (229)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (228)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (247)   

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