KEGG   ENZYME: 1.14.11.82
Entry
EC 1.14.11.82               Enzyme                                 
Name
5-dehydro-6-demethoxyfumagillol dioxygenase;
fmaF (gene name);
Fma-C6H
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
5-dehydro-6-demethoxyfumagillol,2-oxoglutarate:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
5-dehydro-6-demethoxyfumagillol + 2-oxoglutarate + O2 = 5-dehydro-6-demethoxy-6-hydroxyfumagillol + succinate + CO2 [RN:R13122]
Reaction(KEGG)
R13122
Substrate
5-dehydro-6-demethoxyfumagillol [CPD:C22629];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
5-dehydro-6-demethoxy-6-hydroxyfumagillol [CPD:C22631];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires iron(II). The enzyme, characterized from the mold Aspergillus fumigatus, participates in the biosynthesis of the meroterpenoid fumagillin.
History
EC 1.14.11.82 created 2022
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22570  5-dehydro-6-demethoxyfumagillol dioxygenase
Genes
MAJMAA_08347
PCHMVFPPC_11134
AFMAFUA_8G00480
NFINFIA_093990
Reference
1  [PMID:24568283]
  Authors
Lin HC, Tsunematsu Y, Dhingra S, Xu W, Fukutomi M, Chooi YH, Cane DE, Calvo AM, Watanabe K, Tang Y
  Title
Generation of complexity in fungal terpene biosynthesis: discovery of a multifunctional cytochrome P450 in the fumagillin pathway.
  Journal
J Am Chem Soc 136:4426-36 (2014)
DOI:10.1021/ja500881e
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.82
IUBMB Enzyme Nomenclature: 1.14.11.82
ExPASy - ENZYME nomenclature database: 1.14.11.82
BRENDA, the Enzyme Database: 1.14.11.82

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