KEGG ENZYME: 1.14.12.13

ENZYME: 1.14.12.13

Entry

EC 1.14.12.13               Enzyme

Name

2-halobenzoate 1,2-dioxygenase;
2-chlorobenzoate 1,2-dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

2-halobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dehalogenating, decarboxylating)

Reaction(IUBMB)

a 2-halobenzoate + NADH + H+ + O2 = catechol + a halide anion + NAD+ + CO2 [RN:R01033]

Reaction(KEGG)

R01033

Substrate

2-halobenzoate;
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

catechol [CPD:C00090];
halide anion;
NAD+ [CPD:C00003];
CO2 [CPD:C00011]

Comment

A multicomponent enzyme system composed of a dioxygenase component and an electron transfer component. The latter contains FAD. The enzyme, characterized from the bacterium Burkholderia cepacia 2CBS, has a broad substrate specificity. Substrates include 2-fluorobenzoate, 2-chlorobenzoate, 2-bromobenzoate, and 2-iodobenzoate, which are processed in this order of preference.

History

EC 1.14.12.13 created 1992, modified 2012

Pathway

ec00364

Fluorobenzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K08686

2-halobenzoate 1,2-dioxygenase large subunit

K20808

2-halobenzoate 1,2-dioxygenase small subunit

Reference

1 [PMID:2610934]

Authors

Fetzner S, Muller R, Lingens F.

Title

Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS.

Journal

Biol Chem Hoppe Seyler 370:1173-82 (1989)
DOI:10.1515/bchm3.1989.370.2.1173

Reference

2 [PMID:1370284]

Authors

Fetzner S, Muller R, Lingens F

Title

Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS.

Journal

J Bacteriol 174:279-90 (1992)
DOI:10.1128/JB.174.1.279-290.1992

Sequence

[ag:CAA55681 CAA55682]

Reference

3 [PMID:7530709]

Authors

Haak B, Fetzner S, Lingens F

Title

Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS.

Journal

J Bacteriol 177:667-75 (1995)
DOI:10.1128/JB.177.3.667-675.1995

Sequence

[ag:CAA55681 CAA55682]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.12.13

IUBMB Enzyme Nomenclature:

1.14.12.13

ExPASy - ENZYME nomenclature database:

1.14.12.13

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.12.13

BRENDA, the Enzyme Database:

1.14.12.13

CAS:	125268-83-9

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