KEGG ENZYME: 1.14.12.24

ENZYME: 1.14.12.24

Entry

EC 1.14.12.24               Enzyme

Name

2,4-dinitrotoluene dioxygenase;
dntA (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

2,4-dinitrotoluene,NADH:oxygen oxidoreductase (4,5-hydroxylating, nitrite-releasing)

Reaction(IUBMB)

2,4-dinitrotoluene + NADH + O2 = 4-methyl-5-nitrocatechol + nitrite + NAD+ [RN:R09233]

Reaction(KEGG)

R09233

Substrate

2,4-dinitrotoluene [CPD:C11006];
NADH [CPD:C00004];
O2 [CPD:C00007]

Product

4-methyl-5-nitrocatechol [CPD:C18315];
nitrite [CPD:C00088];
NAD+ [CPD:C00003]

Comment

This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.

History

EC 1.14.12.24 created 2015

Pathway

ec00633

Nitrotoluene degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K14579

naphthalene 1,2-dioxygenase subunit alpha

K14580

naphthalene 1,2-dioxygenase subunit beta

Genes

PPI:	ND055(nahAc) ND056(nahAd)

PFK:	PFAS1_28610 PFAS1_28615

PSC:	A458_06720 A458_06725

PSTU:

UIB01_22625 UIB01_22630

PBM:	CL52_14610 CL52_14615

ALT:	ambt_17890 ambt_17895

CYQ:

Q91_2226

CZA:	CYCME_2544

CYY:	CPC19_01670

FCE:	JN531_005655

GBI:	PG2T_11300

RMN:	TK49_07945 TK49_07955

BMK:	DM80_1443(ndoB) DM80_1444(ndoC)

BUQ:	AC233_10850 AC233_11010

BFN:	OI25_3057(ndoB)

PARB:

CJU94_38655 CJU94_38660

PNA:	Pnap_2484 Pnap_2485

AJS:	Ajs_3092 Ajs_3093

ACID:

CBP33_05315

ACIP:

CBP36_05765

ACIN:

CBP34_05150

ACIS:

CBP35_13180

DEL:	DelCs14_1743

MASW:

AM586_27555

RBU:	PG1C_04275 PG1C_06465

NAR:

Saro_3901

NPP:	PP1Y_AT15992 PP1Y_AT31065

NPN:	JI59_12270 JI59_24755

SBAR:

H5V43_22625

SPHY:

CHN51_07270

CNA:	AB433_10400 AB433_18005

ERY:	CP97_14005

» show all

Reference

1 [PMID:8759857]

Authors

Suen WC, Haigler BE, Spain JC

Title

2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase.

Journal

J Bacteriol 178:4926-34 (1996)
DOI:10.1128/JB.178.16.4926-4934.1996

Sequence

[ag:AAB09766 AAB09767]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.12.24

IUBMB Enzyme Nomenclature:

1.14.12.24

ExPASy - ENZYME nomenclature database:

1.14.12.24

BRENDA, the Enzyme Database:

1.14.12.24

DBGET integrated database retrieval system