Entry
Name
benzene 1,2-dioxygenase;
benzene hydroxylase;
benzene dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
benzene + NADH + H+ + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+ [RN:
R03543 ]
Reaction(KEGG)
Substrate
Product
cis-cyclohexa-3,5-diene-1,2-diol [CPD:
C04091 ];
NAD+ [CPD:
C00003 ]
Comment
A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase and ferredoxin. Requires Fe2+.
History
EC 1.14.12.3 created 1972
Pathway
ec00361 Chlorocyclohexane and chlorobenzene degradation
ec01120 Microbial metabolism in diverse environments
Orthology
K03268 benzene/toluene/chlorobenzene dioxygenase subunit alpha
K16268 benzene/toluene/chlorobenzene dioxygenase subunit beta
Genes
PPX : T1E_4266(todC1) T1E_4267(todC2)
PMON : X969_10930 X969_10935
PMOT : X970_10585 X970_10590
PASI : LG197_17450 LG197_17455
ATEM : PQV96_18705 PQV96_18710
Taxonomy
Reference
Authors
Gibson DT, Koch JR, Kallio RE.
Title
Oxidative degradation of aromatic hydrocarbons by microorganisms. I. Enzymatic formation of catechol from benzene.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.12.3
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.3