KEGG ENZYME: 1.14.13.163

ENZYME: 1.14.13.163

Entry

EC 1.14.13.163              Enzyme

Name

6-hydroxy-3-succinoylpyridine 3-monooxygenase;
6-hydroxy-3-succinoylpyridine hydroxylase;
hspA (gene name);
hspB (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

4-(6-hydroxypyridin-3-yl)-4-oxobutanoate,NADH:oxygen oxidoreductase (3-hydroxylating, succinate semialdehyde releasing)

Reaction(IUBMB)

4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 = 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O [RN:R09472]

Reaction(KEGG)

R09472

Substrate

4-(6-hydroxypyridin-3-yl)-4-oxobutanoate [CPD:C19631];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

2,5-dihydroxypyridine [CPD:C01059];
succinate semialdehyde [CPD:C00232];
NAD+ [CPD:C00003];
H2O [CPD:C00001]

Comment

The enzyme catalyses a reaction in the nicotine degradation pathway of Pseudomonas species. One of the enzymes from the soil bacterium Pseudomonas putida S16 contains an FAD cofactor [2].

History

EC 1.14.13.163 created 2012

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K15359

6-hydroxy-3-succinoylpyridine 3-monooxygenase