KEGG ENZYME: 1.14.13.167

ENZYME: 1.14.13.167

Entry

EC 1.14.13.167              Enzyme

Name

4-nitrophenol 4-monooxygenase;
pnpA (gene name);
pdcA (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

4-nitrophenol,NAD(P)H:oxygen 4-oxidoreductase (4-hydroxylating, nitrite-forming)

Reaction(IUBMB)

4-nitrophenol + NADPH + H+ + O2 = 1,4-benzoquinone + nitrite + NADP+ + H2O [RN:R05258]

Reaction(KEGG)

R05258

Substrate

4-nitrophenol [CPD:C00870];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

1,4-benzoquinone [CPD:C00472];
nitrite [CPD:C00088];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

Contains FAD. The enzyme catalyses the first step in a degradation pathway for 4-nitrophenol, the oxidation of 4-nitrophenol at position 4 with the concomitant removal of the nitro group as nitrite. The enzyme from the bacterium Pseudomonas sp. strain WBC-3 also catalyses EC 1.14.13.166, 4-nitrocatechol 4-monooxygenase.

History

EC 1.14.13.167 created 2012

Pathway

ec00627

Aminobenzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K21725

4-nitrocatechol/4-nitrophenol 4-monooxygenase

Genes