KEGG ENZYME: 1.14.13.171

ENZYME: 1.14.13.171

Entry

EC 1.14.13.171              Enzyme

Name

neopentalenolactone D synthase;
ptlE (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (neopentalenolactone-D-forming)

Reaction(IUBMB)

1-deoxy-11-oxopentalenate + NADPH + H+ + O2 = neopentalenolactone D + NADP+ + H2O [RN:R10155]

Reaction(KEGG)

R10155

Substrate

1-deoxy-11-oxopentalenate [CPD:C20400];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

neopentalenolactone D [CPD:C20402];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A FAD-dependent oxygenase. Isolated from the bacterium Streptomyces avermitilis. The ketone undergoes a biological Baeyer-Villiger reaction.

History

EC 1.14.13.171 created 2012

Pathway

ec00998

Biosynthesis of various antibiotics

ec01110

Biosynthesis of secondary metabolites

Orthology

K18091

pentalenolactone D synthase

Genes

MHOL:

K3U96_25175

MHER:

K3U94_10700

SMA:	SAVERM_2994(ptlE)

SBH:

SBI_09676

SCI:	B446_29680

SCW:	TU94_27390

SLE:	sle_13190(sle_13190)

SSIA:

A7J05_09255

SGJ:	IAG43_27140

SHK:	J2N69_35820

Reference

1 [PMID:21250661]

Authors

Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE

Title

Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone.

Journal

Biochemistry 50:1739-54 (2011)
DOI:10.1021/bi1019786

Sequence

[sma:SAVERM_2994]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.171

IUBMB Enzyme Nomenclature:

1.14.13.171

ExPASy - ENZYME nomenclature database:

1.14.13.171

BRENDA, the Enzyme Database:

1.14.13.171

DBGET integrated database retrieval system