KEGG   ENZYME: 1.14.13.18
Entry
EC 1.14.13.18               Enzyme                                 
Name
4-hydroxyphenylacetate 1-monooxygenase;
4-hydroxyphenylacetate 1-hydroxylase;
4-hydroxyphenylacetic 1-hydroxylase;
4-HPA 1-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
4-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (1-hydroxylating)
Reaction(IUBMB)
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 = homogentisate + NAD(P)+ + H2O [RN:R02514 R02516]
Reaction(KEGG)
R02514 R02516
Substrate
4-hydroxyphenylacetate [CPD:C00642];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
homogentisate [CPD:C00544];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). Also acts on 4-hydroxyhydratropate (forming 2-methylhomogentisate) and on 4-hydroxyphenoxyacetate (forming hydroquinone and glycolate).
History
EC 1.14.13.18 created 1976
Pathway
ec00350  Tyrosine metabolism
ec01120  Microbial metabolism in diverse environments
Reference
1  [PMID:234937]
  Authors
Hareland WA, Crawford RL, Chapman PJ, Dagley S.
  Title
Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans.
  Journal
J Bacteriol 121:272-85 (1975)
DOI:10.1128/JB.121.1.272-285.1975
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.18
IUBMB Enzyme Nomenclature: 1.14.13.18
ExPASy - ENZYME nomenclature database: 1.14.13.18
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.18
BRENDA, the Enzyme Database: 1.14.13.18
CAS: 55326-44-8

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (9)   
   KEGG COMPOUND (9)   
Chemical reaction (3)   
   KEGG REACTION (2)   
   KEGG RCLASS (1)   
Protein sequence (3)   
   UniProt (3)   
DNA sequence (14)   
   GenBank (7)   
   EMBL (7)   
Enzyme (1)   
   UMBBD-EC (1)   
All databases (34)   

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