KEGG   ENZYME: 1.14.13.180
Entry
EC 1.14.13.180              Enzyme                                 
Name
aklavinone 12-hydroxylase;
DnrF;
RdmE;
aklavinone 11-hydroxylase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
aklavinone,NADPH:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
aklavinone + NADPH + H+ + O2 = epsilon-rhodomycinone + NADP+ + H2O [RN:R06683]
Reaction(KEGG)
R06683
Substrate
aklavinone [CPD:C12424];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
epsilon-rhodomycinone [CPD:C12425];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzymes from the Gram-positive bacteria Streptomyces peucetius and Streptomyces purpurascens participate in the biosynthesis of daunorubicin, doxorubicin and rhodomycins. The enzyme from Streptomyces purpurascens is an FAD monooxygenase.
History
EC 1.14.13.180 created 2013
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01110  Biosynthesis of secondary metabolites
Orthology
K15950  aklavinone 12-hydroxylase
Genes
SPEUCGZ69_24430
SJNRI060_18375
SKGKJK29_16175
Reference
1  [PMID:7773378]
  Authors
Filippini S, Solinas MM, Breme U, Schluter MB, Gabellini D, Biamonti G, Colombo AL, Garofano L
  Title
Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression.
  Journal
Microbiology 141 ( Pt 4):1007-16 (1995)
DOI:10.1099/13500872-141-4-1007
  Sequence
Reference
2  [PMID:10082933]
  Authors
Niemi J, Wang Y, Airas K, Ylihonko K, Hakala J, Mantsala P
  Title
Characterization of aklavinone-11-hydroxylase from Streptomyces purpurascens.
  Journal
Biochim Biophys Acta 1430:57-64 (1999)
DOI:10.1016/S0167-4838(98)00265-9
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.180
IUBMB Enzyme Nomenclature: 1.14.13.180
ExPASy - ENZYME nomenclature database: 1.14.13.180
BRENDA, the Enzyme Database: 1.14.13.180

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