KEGG ENZYME: 1.14.13.208

ENZYME: 1.14.13.208

Entry

EC 1.14.13.208              Enzyme

Name

benzoyl-CoA 2,3-epoxidase;
benzoyl-CoA dioxygenase/reductase (incorrect);
BoxBA;
BoxA/BoxB system;
benzoyl-CoA 2,3-dioxygenase (incorrect)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

benzoyl-CoA,NADPH:oxygen oxidoreductase (2,3-epoxydizing)

Reaction(IUBMB)

benzoyl-CoA + NADPH + H+ + O2 = 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O [RN:R09555]

Reaction(KEGG)

R09555

Substrate

benzoyl-CoA [CPD:C00512];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

2,3-epoxy-2,3-dihydrobenzoyl-CoA [CPD:C19684];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

The enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii. BoxB functions as the oxygenase part of benzoyl-CoA oxygenase in conjunction with BoxA, the reductase component, which upon binding of benzoyl-CoA, transfers two electrons to the ring in the course of monooxygenation. BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein (FAD), BoxB is a monomeric iron-protein [1].

History

EC 1.14.13.208 created 2010 as EC 1.14.12.21, transferred 2015 to EC 1.14.13.208

Pathway

ec00362

Benzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K15511

benzoyl-CoA 2,3-epoxidase subunit A

K15512

benzoyl-CoA 2,3-epoxidase subunit B

Genes

XAL:	XALC_1517(albXII)

THIS:

HZT40_02635(boxA) HZT40_02640(boxB)

ADI:	B5T_02165 B5T_02166

AXE:	P40_10385 P40_10390

MARL:

HH196_07250(boxB) HH196_07255(boxA)

VFF:	VITFI_CDS1569 VITFI_CDS1589 VITFI_CDS1590

RPJ:	N234_07040 N234_07045 N234_31275 N234_31280

REH:	H16_A1408(bdxA) H16_A1409(bdxB) H16_B1912(boxA) H16_B1913(boxB)

CNC:	CNE_1c14360(boxA) CNE_1c14370(boxB) CNE_2c18730(boxA) CNE_2c18740(boxB) CNE_BB1p04280(boxA)

CUH:	BJN34_07180 BJN34_07185 BJN34_32090 BJN34_32095

REU:	Reut_A1323 Reut_A1324

RME:	Rmet_1220(boxA) Rmet_1221(boxB)

CTI:	RALTA_A1321(bdxA) RALTA_A1322(bdxB) RALTA_B1611(boxA) RALTA_B1612(boxB)

CBW:	RR42_m1517 RR42_m1518 RR42_s3010 RR42_s3011

CGD:	CR3_3379(boxB) CR3_3380(boxA)

CCUP:

BKK81_26785 BKK81_26790

CUP:	BKK80_29525 BKK80_29530

CUU:	BKK79_35480 BKK79_35485

CPAU:

EHF44_15400(boxB) EHF44_15405(boxA)

COX:	E0W60_03165(boxA) E0W60_03170(boxB) E0W60_16990(boxA) E0W60_16995(boxB)

CCAM:

M5D45_06150(boxA) M5D45_06155(boxB)

CCAX:

KZ686_17570(boxA) KZ686_17580(boxB)

CUK:	KB879_07260(boxA) KB879_07265(boxB) KB879_14615(boxA) KB879_14620(boxB)

CNAN:

A2G96_10120 A2G96_10125 A2G96_24425 A2G96_24430

BUG:	BC1001_2389 BC1001_2390

BGF:	BC1003_1068 BC1003_1069

BUQ:	AC233_12235 AC233_12240

BXE:	Bxe_A1423(boxB) Bxe_C0890(boxA) Bxe_C0891(boxB)

BXB:	DR64_3587(boxB) DR64_3588(boxA) DR64_7559(boxB) DR64_7560(boxA)

BPH:	Bphy_1538 Bphy_1539

BGE:	BC1002_1975 BC1002_1976

BPX:	BUPH_02965 BUPH_02966

BPY:	Bphyt_2694 Bphyt_2695

BUZ:	AYM40_13840 AYM40_13845

BFN:	OI25_2716(boxB) OI25_2717(boxA)

BCAI:

K788_0006465 K788_0006466

PSPW:

BJG93_02490(boxB) BJG93_02495(boxA)

PARB:

CJU94_11400(boxB) CJU94_11405(boxA)

PHS:	C2L64_05440(boxB) C2L64_05445(boxA)

PTER:

C2L65_05225(boxB) C2L65_05230(boxA)

PGP:	CUJ91_12135(boxA) CUJ91_12140(boxB)

PCJ:	CUJ87_05135(boxA)

PTS:	CUJ90_12200(boxA) CUJ90_12205(boxB)

PMEG:

FNZ07_26470(boxA) FNZ07_26475(boxB)

PTRO:

G5S35_23230(boxB) G5S35_23235(boxA)

PGIS:

I6I06_01100(boxB) I6I06_01105(boxA)

PACP:

FAZ97_21015(boxA) FAZ97_21020(boxB)

PACS:

FAZ98_17215(boxA) FAZ98_17220(boxB)

PDIO:

PDMSB3_3010(boxA) PDMSB3_3011(boxB)

PBRY:

NDK50_13960(boxA) NDK50_13965(boxB)

PSAA:

QEN71_05370(boxB) QEN71_05375(boxA)

PKF:	RW095_24750(boxB) RW095_24755(boxA)

PPAI:

E1956_16985(boxB) E1956_16990(boxA)

BRH:	RBRH_00498 RBRH_00499

PPK:	U875_06785 U875_06790

PPNO:

DA70_23860 DA70_23865

PPNM:

LV28_06845 LV28_06850

PRB:	X636_02785 X636_02790

PPUL:

RO07_00930 RO07_00935

PSPU:

NA29_10730 NA29_10735

PAPI:

SG18_01105 SG18_01110

PVE:	UC34_17185 UC34_17190

POX:	MB84_21960 MB84_21965

PTX:	ABW99_07615 ABW99_07620

PFG:	AB870_04225 AB870_04230

PNR:	AT302_14910 AT302_14915

PAND:

DRB87_15890(boxB)

PFIB:

PI93_010795(boxB) PI93_010800(boxA)

PCOM:

NTU39_18885(boxA) NTU39_18890(boxB)

TVL:	FAZ95_11600(boxA) FAZ95_11605(boxB)

BPT:	Bpet3568(petH) Bpet3569(pbcA)

BHO:	D560_2021(boxB) D560_2022(boxA)

BHM:	D558_2006(boxB) D558_2007(boxA)

BHZ:	ACR54_04083(boxA) ACR54_04084(boxB)

BBRO:

BAU06_05180 BAU06_05185

BFZ:	BAU07_21240 BAU07_21245

BPDZ:

BBN53_02670 BBN53_02675

BGM:	CAL15_08655 CAL15_08660

BGV:	CAL12_05290 CAL12_05295

AXY:	AXYL_05030(boxA) AXYL_05031(boxB)

AXO:	NH44784_035141 NH44784_035151

AXN:	AX27061_4849 AX27061_4850

AXX:	ERS451415_04886(boxA) ERS451415_04887(boxB)

ADT:	APT56_22175 APT56_22180

AIS:	BUW96_13975 BUW96_13980

ASW:	CVS48_13535(boxB) CVS48_13540(boxA)

ACHB:

DVB37_21270(boxA) DVB37_21275(boxB)

ADY:	HLG70_28660(boxB) HLG70_28665(boxA)

ACHO:

H4P35_20150(boxA) H4P35_20155(boxB)

AMUI:

PE062_16805(boxA) PE062_16810(boxB)

APES:

FOC84_20830(boxB) FOC84_20835(boxA)

AAEG:

RA224_07450(boxB) RA224_07455(boxA)

PUD:	G9Q38_06775(boxB) G9Q38_06780(boxA)

PUS:	CKA81_01055(boxB) CKA81_01060(boxA)

POLL:

OEG81_13620(boxA) OEG81_13625(boxB)

AKA:	TKWG_07590 TKWG_07595

AMIM:

MIM_c27160(boxA) MIM_c27170(boxB)

CDN:	BN940_14751 BN940_14756

PIG:	EGT29_17285(boxA) EGT29_17290(boxB)

PARK:

LSG25_10585(boxA) LSG25_10590(boxB)

NARM:

N7E01_12350(boxB)

RFR:	Rfer_0221 Rfer_0222 Rfer_0228

RHF:	EUB48_04950(boxB) EUB48_04955(boxA)

POL:	Bpro_1629 Bpro_1630

PNA:	Pnap_2942 Pnap_2943

POS:	DT070_17675(boxB)

POO:	F7R28_12910(boxB)

PVAC:

HC248_01624(boxB)

ACK:	C380_20165 C380_20170

VEI:	Veis_0733 Veis_0734

DAC:	Daci_0072 Daci_0073

DEL:	DelCs14_0073 DelCs14_0074

DTS:	BI380_22090 BI380_22095

DHK:	BO996_02630 BO996_02635

DLA:	I6G47_22040(boxA) I6G47_22045(boxB)

VAP:	Vapar_0094 Vapar_0095

VPE:	Varpa_0110 Varpa_0111

VPD:	VAPA_1c00960(boxB) VAPA_1c00970(boxA)

VBO:	CKY39_00545(boxB) CKY39_00550(boxA)

VAM:	C4F17_18550(boxA) C4F17_18555(boxB)

CTT:	CtCNB1_0065 CtCNB1_0066

CTES:

O987_00305 O987_00310

CRJ:	QMY55_00230(boxA) QMY55_00235(boxB)

RTA:

Rta_22300

OTD:	J1M35_05285(boxB) J1M35_05290(boxA)

LIM:	L103DPR2_00830(boxB) L103DPR2_00831(boxA)

LIH:	L63ED372_02245(boxA) L63ED372_02246(boxB)

HYR:	BSY239_635(boxB) BSY239_636(boxA)

HYB:	Q5W_16380 Q5W_16385

HYL:	LPB072_05890 LPB072_05895

HYC:	E5678_00695(boxB) E5678_00700(boxA)

HPSE:

HPF_06705(boxB) HPF_06710(boxA)

HYN:	F9K07_05880(boxB) F9K07_05885(boxA)

HTN:	KI616_00735(boxB) KI616_07625(boxB) KI616_07630(boxA) KI616_12830(boxB) KI616_12835(boxA)

HCZ:	G9Q37_21450(boxB) G9Q37_21455(boxA)

DIH:	G7047_17820(boxB) G7047_17825(boxA)

DAER:

H9K75_07770(boxA)

DRG:	H9K76_04120(boxA) H9K76_04125(boxB)

CBAA:

SRAA_0585(boxB) SRAA_0586(boxA)

SAQA:

OMP39_14385(boxB) OMP39_14390(boxA)

SNN:	EWH46_00460(boxB) EWH46_00465(boxA)

ATER:

MW290_08050(boxA) MW290_08055(boxB)

LCH:	Lcho_3660 Lcho_3661

THI:	THI_0466(boxA) THI_0467(boxB)

SHD:	SUTH_01641 SUTH_01642(boxB)

EBA:	ebA2765(boxB) ebA2766(boxA)

ABRE:

pbN1_17410(boxB) pbN1_17420(boxA)

APET:

ToN1_26650(boxA) ToN1_26660(boxB)

AZO:	azo3057(boxB) azo3058(boxA)

AOA:	dqs_3191 dqs_3192

AZA:	AZKH_4519(boxB) AZKH_4520(boXA)

AZI:	AzCIB_4637 AzCIB_4638

AZD:	CDA09_22740(boxB) CDA09_22745(boxA)

AZR:	CJ010_18910(boxB) CJ010_18915(boxA)

TMZ:	Tmz1t_3908

TCL:	Tchl_1102 Tchl_1103

TAK:	Tharo_1133 Tharo_1134

DDZ:	DSYM_19390 DSYM_19400

MESO:

BSQ44_18020 BSQ44_18030

RPOD:

E0E05_11135(boxA) E0E05_11140(boxB)

NIY:	FQ775_02915(boxA) FQ775_02920(boxB)

KMN:	HW532_10735(boxB) HW532_10740(boxA)

ABAW:

D5400_06470(boxB) D5400_06475(boxA)

BJA:	blr1081(blr1081)

BJU:	BJ6T_11110

BJP:	RN69_05425

BRA:	BRADO6788(boxB)

BBT:	BBta_0750(boxB) BBta_6638(boxA) BBta_6639(boxB)

BRS:

S23_67350

AOL:

S58_06720

BRC:	BCCGELA001_34210

BIC:	LMTR13_36350

BRO:	BRAD285_6936(boxB)

BRK:	CWS35_10185(boxB)

BOT:	CIT37_08870(boxB)

BRQ:	CIT40_30010(boxB)

BGQ:	X265_34635(boxB)

BGZ:	XH91_33020(boxB)

BSYM:

CIT39_29125(boxB)

BBET:

F8237_13005(boxB)

BARH:

WN72_46140(boxB)

BVZ:	BRAD3257_8645(boxB)

BEL:	BE61_90290(boxB)

BDG:	LPJ38_02110(boxB)

BSEP:

HAP48_0011930(boxB)

BQB:	J4P68_0015770(boxB)

BSEI:

KMZ68_03460(boxB) KMZ68_03465(boxA) KMZ68_03470(boxB)

BBAN:

J4G43_003790(boxB)

BCAN:

BcanWSM471_35425(boxB)

BAUT:

QA635_04860(boxB)

BBRA:

QA636_04335(boxB)

BGK:	IC762_32485(boxB)

BCOU:

IC761_34605(boxB)

BPAH:

QA639_04280(boxB)

BXN:	I3J27_37555(boxB)

BROS:

QUH67_33645(boxB)

BRAZ:

LRP30_42130(boxB)

RPA:	TX73_003505(boxB)

RPB:

RPB_4653

RPD:

RPD_0881

RPE:

RPE_0584

RPT:

Rpal_0744

RPX:	Rpdx1_4109

RBK:	E0H22_11695(boxB) E0H22_11700(boxA)

VGO:	GJW-30_1_01040(boxB) GJW-30_1_01041(boxA)

TRB:	HB776_18400(boxA) HB776_18405(boxB)

RHZ:	RHPLAN_60810

PSIN:

CAK95_09715 CAK95_09720

RBM:

TEF_07960

CAUL:

KCG34_06220(boxB)

SIL:	SPO3701(boxB) SPO3703(boxA)

JAN:	Jann_0672(boxB)

RPON:

G3256_16600(boxA) G3256_16605(boxB)

SUAM:

BOO69_15565 BOO69_15570

SULZ:

C1J03_02545(boxB) C1J03_02555(boxA)

SMED:

JNX03_11395(boxA) JNX03_11400(boxB)

SPSE:

SULPSESMR1_02743(boxA) SULPSESMR1_02745(boxB)

TOM:	BWR18_14110

RID:	RIdsm_00626(boxB) RIdsm_00628(boxA)

AHT:	ANTHELSMS3_04341(boxA) ANTHELSMS3_04343(boxB)

SAGU:

CDO87_17735(boxB) CDO87_17745(boxA)

THAA:

CFI11_22010(boxB)

OCD:	FHY55_19195(boxA) FHY55_19200(boxB)

MALU:

KU6B_38040 KU6B_38050

TGL:	HFZ77_06780(boxA) HFZ77_06790(boxB)

TRY:	QF118_10495(boxB) QF118_10505(boxA)

PZH:	CX676_12415(boxA)

PTP:	RCA23_c19060(boxA) RCA23_c19070(boxB)

SEDI:

EBB79_19920(boxA) EBB79_19925(boxB)

PPSO:

QPJ95_08525(boxB) QPJ95_08535(boxA)

GCE:	KYE46_09685(boxB) KYE46_09695(boxA)

MAGX:

XM1_1070(boxB) XM1_1071(boxA)

MAGN:

WV31_13525 WV31_13530

FER:	FNB15_18640(boxB)

MAG:	amb0175 amb0176

NACI:

NUH88_09980(boxB)

SNEA:

NBZ79_15020(boxB)

APAU:

AMPC_37390

SUR:	STAUR_7626(boxB)

AVM:	JQX13_24540(boxB)

VIN:	AKJ08_1016

SCL:	sce1060(yfcX)

SCU:	SCE1572_07645

CCRO:

CMC5_025770 CMC5_025780

PAUU:

E8A73_043340(boxB)

MRM:	A7982_06572

SAMY:

DB32_002623

LLU:	AKJ09_05632

HOH:

Hoch_5225

NANN:

O0S08_50210(boxB)

MFJ:	MFLOJ_46030

MLW:	MJO58_22405(boxB)

MMOR:

MMOR_02900

RANT:

RHODO2019_00955(boxB)

GOQ:	ACH46_13370

GOI:	LK459_15645(boxB)

TOY:	FO059_00815(boxB)

SBAT:

G4Z16_02410(boxB)

SSPN:

LXH13_30045(boxB)

JLI:	EXU32_11305(boxB)

JCR:	O9K63_16250(boxB)

NPS:	KRR39_16265(boxB)

SAIU:

J4H86_15070(boxB)

AMAZ:

LUW76_06990(boxB)

ACIR:

OG979_34110(boxB)

ACTQ:

OG417_22045(boxB)

NOA:	BKM31_25130

NGN:	LCN96_23775(boxB)

NFS:	OIE67_25000(boxB)

BSD:	BLASA_2556(boxB)

AMD:

AMED_8610

AMN:

RAM_44190

AMM:

AMES_8479

AMZ:

B737_8480

AOI:

AORI_7396

AJA:	AJAP_02020(boxB)

AMQ:	AMETH_6479

AMYC:

CU254_35735(boxB)

AMYB:

BKN51_30385

AMYY:

YIM_21145(boxB)

AORI:

SD37_03480

AACD:

LWP59_36010(boxB)

APRT:

MUY14_40655(boxB)

AROO:

NQK81_28605(boxB)

ATHM:

L1857_34450(boxB)

ARHD:

VSH64_04775(boxB) VSH64_32955(boxB)

PDX:

Psed_1489

PSEA:

WY02_22245

PSEE:

FRP1_20445

PSEH:

XF36_05450

PSEQ:

AD006_27860

PECQ:

AD017_07455

PHH:	AFB00_18000

PAUT:

Pdca_13940

PBRO:

HOP40_01905(boxB)

PPEL:

H6H00_02555(boxB)

KUT:	JJ691_02600

KPHY:

AOZ06_19790

PMAD:

BAY61_05970

ACTY:

OG774_01100(boxB)

CAI:

Caci_2007

EKE:	EK0264_08340(boxB)

VAB:

WPS_02270

» show all

Reference

1 [PMID:15458418]

Authors

Zaar A, Gescher J, Eisenreich W, Bacher A, Fuchs G

Title

New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii.

Journal

Mol Microbiol 54:223-38 (2004)
DOI:10.1111/j.1365-2958.2004.04263.x

Sequence

[ag:AAN39377 AAN39376]

Reference

2 [PMID:12399500]

Authors

Gescher J, Zaar A, Mohamed M, Schagger H, Fuchs G

Title

Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii.

Journal

J Bacteriol 184:6301-15 (2002)
DOI:10.1128/JB.184.22.6301-6315.2002

Sequence

[ag:AAN39377 AAN39376]

Reference

3 [PMID:11222587]

Authors

Mohamed ME, Zaar A, Ebenau-Jehle C, Fuchs G

Title

Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii.

Journal

J Bacteriol 183:1899-908 (2001)
DOI:10.1128/JB.183.6.1899-1908.2001

Reference

4 [PMID:20452977]

Authors

Rather LJ, Knapp B, Haehnel W, Fuchs G

Title

Coenzyme A-dependent aerobic metabolism of benzoate via epoxide formation.

Journal

J Biol Chem 285:20615-24 (2010)
DOI:10.1074/jbc.M110.124156

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.208

IUBMB Enzyme Nomenclature:

1.14.13.208

ExPASy - ENZYME nomenclature database:

1.14.13.208

BRENDA, the Enzyme Database:

1.14.13.208

DBGET integrated database retrieval system