KEGG ENZYME: 1.14.13.210

ENZYME: 1.14.13.210

Entry

EC 1.14.13.210              Enzyme

Name

4-methyl-5-nitrocatechol 5-monooxygenase;
dntB (gene name);
4-methyl-5-nitrocatechol oxygenase;
MNC monooxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

4-methyl-5-nitrocatechol,NAD(P)H:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)

Reaction(IUBMB)

4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O [RN:R09234 R11150]

Reaction(KEGG)

R09234 R11150

Substrate

4-methyl-5-nitrocatechol [CPD:C18315];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

2-hydroxy-5-methylquinone [CPD:C18316];
nitrite [CPD:C00088];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

Contains FAD. The enzyme, isolated from the bacterium Burkholderia sp. DNT, can use both NADH and NADPH, but prefers NADPH. It has a narrow substrate range, but can also act on 4-nitrocatechol.

History

EC 1.14.13.210 created 2016

Pathway

ec00633

Nitrotoluene degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K21683

4-methyl-5-nitrocatechol 5-monooxygenase

Reference

1 [PMID:8830701]