KEGG   ENZYME: 1.14.13.212
Entry
EC 1.14.13.212              Enzyme                                 
Name
1,3,7-trimethyluric acid 5-monooxygenase;
tmuM (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate-forming)
Reaction(IUBMB)
1,3,7-trimethylurate + NADH + H+ + O2 = 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O [RN:R11153]
Reaction(KEGG)
R11153
Substrate
1,3,7-trimethylurate [CPD:C16361];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
1,3,7-trimethyl-5-hydroxyisourate [CPD:C21144];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase.
History
EC 1.14.13.212 created 2016
Pathway
ec00232  Caffeine metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K00492  1,3,7-trimethyluric acid 5-monooxygenase
Reference
1  [PMID:22609920]
  Authors
Mohanty SK, Yu CL, Das S, Louie TM, Gakhar L, Subramanian M
  Title
Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism.
  Journal
J Bacteriol 194:3872-82 (2012)
DOI:10.1128/JB.00597-12
  Sequence
[ag:AFO10115]
Reference
2  [PMID:25678373]
  Authors
Summers RM, Mohanty SK, Gopishetty S, Subramanian M
  Title
Genetic characterization of caffeine degradation by bacteria and its potential applications.
  Journal
Microb Biotechnol 8:369-78 (2015)
DOI:10.1111/1751-7915.12262
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.212
IUBMB Enzyme Nomenclature: 1.14.13.212
ExPASy - ENZYME nomenclature database: 1.14.13.212
BRENDA, the Enzyme Database: 1.14.13.212

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
All databases (15)   

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