KEGG ENZYME: 1.14.13.217

ENZYME: 1.14.13.217

Entry

EC 1.14.13.217              Enzyme

Name

protodeoxyviolaceinate monooxygenase;
vioD (gene name);
protoviolaceinate synthase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

protodeoxyviolaceinate,NAD(P)H:O2 oxidoreductase

Reaction(IUBMB)

protodeoxyviolaceinate + NAD(P)H + O2 = protoviolaceinate + NAD(P)+ + H2O [RN:R11134 R11158]

Reaction(KEGG)

R11134 R11158

Substrate

protodeoxyviolaceinate [CPD:C21131];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007]

Product

protoviolaceinate [CPD:C21134];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The product, protoviolaceinate, can be acted upon by EC 1.14.13.224, violacein synthase, leading to violacein production. However, it is very labile, and in the presence of oxygen can undergo non-enzymic autooxidation to the shunt product proviolacein.

History

EC 1.14.13.217 created 2016, modified 2016

Pathway

ec00404

Staurosporine biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K20089

protodeoxyviolaceinate monooxygenase

Genes

CVI:	CV_3271(vioD)

CVC:	BKX93_07100

CHRI:

DK842_01225

CHRB:

DK843_06505

CHRM:

FYK34_10580

JAG:	GJA_1083(vioD)

JAZ:	YQ44_10555

JAL:	BZG29_16960

JLV:	G3257_17690(vioD)

JAH:	JAB4_031350(vioD)

JRI:	P9875_17645

MNR:	ACZ75_19205

MASS:

CR152_21620

MANC:

IV454_26320

RIV:	Riv7116_3967

» show all

Reference

1 [PMID:17176066]

Authors

Balibar CJ, Walsh CT

Title

In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.

Journal

Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z

Reference

2 [PMID:17925955]

Authors

Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T.

Title

Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.

Journal

10.1039/b705358d

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.217

IUBMB Enzyme Nomenclature:

1.14.13.217

ExPASy - ENZYME nomenclature database:

1.14.13.217

BRENDA, the Enzyme Database:

1.14.13.217

DBGET integrated database retrieval system