KEGG ENZYME: 1.14.13.224

ENZYME: 1.14.13.224

Entry

EC 1.14.13.224              Enzyme

Name

violacein synthase;
proviolaceinate monooxygenase;
vioC (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

protoviolaceinate,NAD(P)H:O2 oxidoreductase

Reaction(IUBMB)

(1) protoviolaceinate + NAD(P)H + O2 = violaceinate + NAD(P)+ + H2O [RN:R11135 R11373];
(2) protodeoxyviolaceinate + NAD(P)H + O2 = deoxyviolaceinate + NAD(P)+ + H2O [RN:R11132 R11374]

Reaction(KEGG)

R11132 R11135 R11373 R11374

Substrate

protoviolaceinate [CPD:C21134];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007];
protodeoxyviolaceinate [CPD:C21131]

Product

violaceinate [CPD:C21135];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
deoxyviolaceinate [CPD:C21132]

Comment

The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The products, violaceinate and deoxyviolaceinate, undergo non-enzymic autooxidation into violacein and deoxyviolacein, respectively.

History

EC 1.14.13.224 created 2016

Pathway

ec00404

Staurosporine biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K20090

violacein synthase

Genes

CVI:	CV_3272(vioC)

CVC:	BKX93_07105

CHRI:

DK842_01230

CHRB:

DK843_06510

CHRM:

FYK34_10585

IFL:	C1H71_10080

JAG:	GJA_1082(vioC)

JAZ:	YQ44_10560

JAL:	BZG29_16955

JLV:	G3257_17685(vioC)

JAH:	JAB4_031360(vioC)

JRI:	P9875_17640

MNR:	ACZ75_19210

MASS:

CR152_21625

MANC:

IV454_26315

MSD:	MYSTI_01836

RIV:	Riv7116_3968

» show all

Reference

1 [PMID:17176066]

Authors

Balibar CJ, Walsh CT

Title

In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.

Journal

Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z

Reference

2 [PMID:17925955]

Authors

Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T.

Title

Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.

Journal

10.1039/b705358d

Sequence

[cvi:CV_3272]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.224

IUBMB Enzyme Nomenclature:

1.14.13.224

ExPASy - ENZYME nomenclature database:

1.14.13.224

BRENDA, the Enzyme Database:

1.14.13.224

DBGET integrated database retrieval system