KEGG   ENZYME: 1.14.13.23
Entry
EC 1.14.13.23               Enzyme                                 
Name
3-hydroxybenzoate 4-monooxygenase;
3-hydroxybenzoate 4-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
3-hydroxybenzoate + NADPH + H+ + O2 = 3,4-dihydroxybenzoate + NADP+ + H2O [RN:R01628]
Reaction(KEGG)
R01628
Substrate
3-hydroxybenzoate [CPD:C00587];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,4-dihydroxybenzoate [CPD:C00230];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions.
History
EC 1.14.13.23 created 1972 as EC 1.14.99.13, transferred 1984 to EC 1.14.13.23
Pathway
ec00362  Benzoate degradation
ec00624  Polycyclic aromatic hydrocarbon degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K19065  3-hydroxybenzoate 4-monooxygenase
Genes
CCAGSR908_06545
RSBRS694_04110
RHYRD110_25690
CTTCtCNB1_3410
CTESO987_20190
HYNF9K07_22880
MLIRLPB04_03180
PSANHGN31_07305
GPRJQN66_14315
BSPOL1F31_18425
Reference
1  [PMID:4148586]
  Authors
Michalover JL, Ribbons DW, Hughes H.
  Title
3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni.
  Journal
Biochem Biophys Res Commun 55:888-96 (1973)
DOI:10.1016/0006-291X(73)91227-8
Reference
2  [PMID:4390252]
  Authors
Premkumar R, Rao PV, Sreeleela NS, Vaidyanathan CS.
  Title
m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger.
  Journal
Can J Biochem 47:825-7 (1969)
DOI:10.1139/o69-129
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.23
IUBMB Enzyme Nomenclature: 1.14.13.23
ExPASy - ENZYME nomenclature database: 1.14.13.23
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.23
BRENDA, the Enzyme Database: 1.14.13.23
CAS: 37256-76-1

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