KEGG   ENZYME: 1.14.13.230
Entry
EC 1.14.13.230              Enzyme                                 
Name
butane monooxygenase (soluble);
sBMO;
bmoBCDXYZ (gene names)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
butane,NADH:oxygen oxidoreductase
Reaction(IUBMB)
butane + NADH + H+ + O2 = butan-1-ol + NAD+ + H2O [RN:R11448]
Reaction(KEGG)
R11448
Substrate
butane [CPD:C21390];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
butan-1-ol [CPD:C06142];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Thauera butanivorans, is similar to EC 1.14.13.25, methane monooxygenase (soluble), but has a very low activity with methane. It comprises three components - a carboxylate-bridged non-heme di-iron center-containing hydroxylase (made of three different subunits), a flavo-iron sulfur-containing NADH-oxidoreductase, and a small regulatory component protein. The enzyme can also act on other C3-C6 linear and branched aliphatic alkanes with lower activity.
History
EC 1.14.13.230 created 2016
Pathway
ec00650  Butanoate metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K21317  butane monooxygenase alpha subunit
K21318  butane monooxygenase beta subunit
K21319  butane monooxygenase gamma subunit
Genes
AZRCJ010_10840 CJ010_10850 CJ010_10855
MMEHM5I08_04105
MHOLK3U96_21115
Reference
1  [PMID:12427952]
  Authors
Sluis MK, Sayavedra-Soto LA, Arp DJ
  Title
Molecular analysis of the soluble butane monooxygenase from 'Pseudomonas butanovora'.
  Journal
Microbiology 148:3617-29 (2002)
DOI:10.1099/00221287-148-11-3617
  Sequence
Reference
2  [PMID:17526838]
  Authors
Dubbels BL, Sayavedra-Soto LA, Arp DJ
  Title
Butane monooxygenase of 'Pseudomonas butanovora': purification and biochemical characterization of a terminal-alkane hydroxylating diiron monooxygenase.
  Journal
Microbiology 153:1808-16 (2007)
DOI:10.1099/mic.0.2006/004960-0
Reference
3  [PMID:18281403]
  Authors
Doughty DM, Kurth EG, Sayavedra-Soto LA, Arp DJ, Bottomley PJ
  Title
Evidence for involvement of copper ions and redox state in regulation of butane monooxygenase in Pseudomonas butanovora.
  Journal
J Bacteriol 190:2933-8 (2008)
DOI:10.1128/JB.01409-07
Reference
4  [PMID:19383682]
  Authors
Cooley RB, Dubbels BL, Sayavedra-Soto LA, Bottomley PJ, Arp DJ
  Title
Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly 'Pseudomonas butanovora'.
  Journal
Microbiology 155:2086-96 (2009)
DOI:10.1099/mic.0.028175-0
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.230
IUBMB Enzyme Nomenclature: 1.14.13.230
ExPASy - ENZYME nomenclature database: 1.14.13.230
BRENDA, the Enzyme Database: 1.14.13.230

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