KEGG   ENZYME: 1.14.13.233
Entry
EC 1.14.13.233              Enzyme                                 
Name
4-hydroxy-6-methylpretetramide 12a-monooxygenase;
oxyL (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
4-hydroxy-6-methylpretetramide,NADPH:oxygen oxidoreductase (12a-hydroxylating)
Reaction(IUBMB)
4-hydroxy-6-methylpretetramide + NADPH + H+ + O2 = 4-de(dimethylamino)-4-oxoanhydrotetracycline + NADP+ + H2O [RN:R05462]
Reaction(KEGG)
R05462;
(other) R09190
Substrate
4-hydroxy-6-methylpretetramide [CPD:C06628];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
4-de(dimethylamino)-4-oxoanhydrotetracycline [CPD:C06627];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Contains FAD. The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. The enzyme is bifunctional, and can also catalyse EC 1.14.13.232, 6-methylpretetramide 4-monooxygenase.
History
EC 1.14.13.233 created 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14252  6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
Genes
NAH: F5544_10695
SHY: SHJG_1193
SHO: SHJGH_1028
SBH: SBI_06866
SCI: B446_13470
SPRI: SPRI_0201 SPRI_7152
SPAV: Spa2297_32760
SGS: AVL59_46185
SGM: GCM10017557_73940
SPHW: NFX46_25010
SGZ: C0216_31875
SVR: CP971_24005
SGF: HEP81_07938
SRIM: CP984_02425
SPLA: CP981_34475
SHUN: DWB77_05143(pcpB_4)
SXN: IAG42_36430
SINE: KI385_25240
SYAN: NRK68_34850
SVIO: HWN34_29125
SSPN: LXH13_14080
SDRZ: NEH16_32465
SHAU: K9S39_12920
KAU: B6264_18580
KIS: HUT16_23740
MIK: FOE78_19175
AORI: SD37_34185
ARHD: VSH64_28950
KAL: KALB_7834
KUT: JJ691_15620
ACTU: Actkin_02482(pcpB_1)
CROS: N8J89_20350
PLK: CIK06_11100
DAUR: Daura_37895
CCAZ: COUCH_15955
KBB: ccbrp13_21970
 » show all
Reference
1  [PMID:18422316]
  Authors
Zhang W, Watanabe K, Cai X, Jung ME, Tang Y, Zhan J
  Title
Identifying the minimal enzymes required for anhydrotetracycline biosynthesis.
  Journal
J Am Chem Soc 130:6068-9 (2008)
DOI:10.1021/ja800951e
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.233
IUBMB Enzyme Nomenclature: 1.14.13.233
ExPASy - ENZYME nomenclature database: 1.14.13.233
BRENDA, the Enzyme Database: 1.14.13.233

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Pathway (10)   
   KEGG PATHWAY (8)   
   KEGG MODULE (2)   
Chemical substance (8)   
   KEGG COMPOUND (8)   
Chemical reaction (4)   
   KEGG REACTION (2)   
   KEGG RCLASS (2)   
Gene (39)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (38)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (3)   
   InterPro (3)   
All databases (66)   

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