Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

L-aspartate,NADPH:oxygen oxidoreductase [(2S)-2-nitrobutanedioate-forming]

L-aspartate + 3 NADPH + 3 H+ + 3 O2 = (2S)- 2-nitrobutanedioate + 3 NADP+ + 4 H2O [RN:R12907];
(1a) L-aspartate + NADPH + H+ + O2 = N-hydroxy-L-aspartate + NADP+ + H2O [RN:R12908];
(1b) N-hydroxy-L-aspartate + NADPH + H+ + O2 = N,N-dihydroxy-L-aspartate + NADP+ + H2O [RN:R12909];
(1c) N,N-dihydroxy-L-aspartate = (2S)-2-nitrosobutanedioate + H2O (spontaneous) [RN:R12910];
(1d) (2S)-2-nitrosobutanedioate + NADPH + H+ + O2 = (2S)-2-nitrobutanedioate + NADP+ + H2O [RN:R12911]

R12907 R12908 R12909 R12910 R12911
Reaction

L-aspartate [CPD:C00049];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
N-hydroxy-L-aspartate [CPD:C22380];
N,N-dihydroxy-L-aspartate [CPD:C22333];
(2S)-2-nitrosobutanedioate [CPD:C22334]

The enzyme, found in some Actinobacteria, is involved in a pathway that forms nitrite, which is subsequently used to generate a diazo group in some secondary metabolites. Requires an FAD cofactor.

EC 1.14.13.248 created 2021

1  [PMID:26689788]

2  [PMID:30120394]