KEGG ENZYME: 1.14.14.102

ENZYME: 1.14.14.102

Entry

EC 1.14.14.102              Enzyme

Name

N-methylcoclaurine 3'-monooxygenase;
N-methylcoclaurine 3'-hydroxylase;
CYP80B1 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

(S)-N-methylcoclaurine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)

Reaction(IUBMB)

(S)-N-methylcoclaurine + [reduced NADPH---hemoprotein reductase] + O2 = (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R05732]

Reaction(KEGG)

R05732

Substrate

(S)-N-methylcoclaurine [CPD:C05176];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

(S)-3'-hydroxy-N-methylcoclaurine [CPD:C05202];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein involved in benzylisoquinoline alkaloid synthesis in higher plants.

History

EC 1.14.14.102 created 2001 as 1.14.13.71, transferred 2018 to EC 1.14.14.102

Pathway

ec00950

Isoquinoline alkaloid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K13385

N-methylcoclaurine 3'-monooxygenase

Genes

PSOM:

113314340

Reference

1 [PMID:9681018]

Authors

Pauli HH, Kutchan TM

Title

Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis.

Journal

Plant J 13:793-801 (1998)
DOI:10.1046/j.1365-313X.1998.00085.x

Sequence

[ag:AAC39452 AAC39453]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.102

IUBMB Enzyme Nomenclature:

1.14.14.102

ExPASy - ENZYME nomenclature database:

1.14.14.102

BRENDA, the Enzyme Database:

1.14.14.102

CAS:	202420-37-9

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