KEGG ENZYME: 1.14.14.111

ENZYME: 1.14.14.111

Entry

EC 1.14.14.111              Enzyme

Name

9beta-pimara-7,15-diene oxidase;
CYP99A3;
9beta-pimara-7,15-diene monooxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

9beta-pimara-7,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen 19-oxidoreductase

Reaction(IUBMB)

9beta-pimara-7,15-diene + 3 O2 + 3 [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R09865];
(1a) 9beta-pimara-7,15-diene + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09862];
(1b) 9beta-pimara-7,15-dien-19-ol + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R09863];
(1c) 9beta-pimara-7,15-dien-19-al + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09864]

Reaction(KEGG)

R09862 R09863 R09864 R09865;
(other) R09921

Substrate

9beta-pimara-7,15-diene [CPD:C18225];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]

Product

9beta-pimara-7,15-dien-19-oate [CPD:C20149];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]

Comment

A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the phytoalexin momilactone. It also acts similarly on 9beta-stemod-13(17)-ene.

History

EC 1.14.14.111 created 2012 as EC 1.14.13.144, transferred 2018 to EC 1.14.14.111

Pathway

ec00904

Diterpenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K16085

9beta-pimara-7,15-diene oxidase

Genes

OSA:	4335091 4335096

DOSA:

Os04t0178400-01(Os04g0178400) Os04t0180400-01(Os04g0180400)

OBR:	102705642 102721444

OGL:	127769969 127770008

BDI:

100836875

ATS:	109731407 109734305 109743692 109750535 109773965 109773967 109773970 109774538 109776121 109780586 109780589

TDC:	119271910 119272669 119278132 119281969 119351866 119356226 119358650 119368198

TAES:

123041873 123041878 123043471 123046476 123048254 123048256 123048257 123048258 123049849 123049853 123054377 123055487 123057860 123063080 123064868 123071979 123074029 123075233 123100267 123126305 123169592 123184294 123184298 123184300 123184301 123184302 123185921 123185949 123190208

TUA:	125528121 125533911 125533928 125540541 125540674 125547908 125549032

LPER:

127292356 127318419 127336229 127336234 127337247 127338821 127345412

LRD:	124651229 124667729 124667960 124668042 124672078 124672080 124676243 124677570 124679047 124706022 124706208

SBI:	8069842 8080269 8081807 8085991

ZMA:	100273010 103641402 103652787

SITA:

101767767 101772065 101772739 101776139 101776959

SVS:	117841995 117842511 117859811 117860031 117860109 117865140

PVIR:

120651251 120660277 120674828 120690916

PHAI:

112879668 112896256

» show all

Reference

1 [PMID:21175892]

Authors

Wang Q, Hillwig ML, Peters RJ

Title

CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice.

Journal

Plant J 65:87-95 (2011)
DOI:10.1111/j.1365-313X.2010.04408.x

Sequence

[osa:4335091]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.111

IUBMB Enzyme Nomenclature:

1.14.14.111

ExPASy - ENZYME nomenclature database:

1.14.14.111

BRENDA, the Enzyme Database:

1.14.14.111

DBGET integrated database retrieval system