KEGG ENZYME: 1.14.14.112

ENZYME: 1.14.14.112

Entry

EC 1.14.14.112              Enzyme

Name

ent-cassa-12,15-diene 11-hydroxylase;
ent-cassadiene C11alpha-hydroxylase;
CYP76M7

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

ent-cassa-12,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen 11-oxidoreductase

Reaction(IUBMB)

ent-cassa-12,15-diene + O2 + [reduced NADPH---hemoprotein reductase] = ent-11beta-hydroxycassa-12,15-diene + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09866]

Reaction(KEGG)

R09866

Substrate

ent-cassa-12,15-diene [CPD:C18226];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]

Product

ent-11beta-hydroxycassa-12,15-diene [CPD:C20150];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the antifungal phytocassanes.

History

EC 1.14.14.112 created 2012 as EC 1.14.13.145, transferred 2018 to EC 1.14.14.112

Pathway

ec00904

Diterpenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K16084

ent-cassa-12,15-diene 11-hydroxylase / oryzalexin D synthase

Genes

OSA:	4329722 4329723 4341447

DOSA:

Os02t0569400-01(Os02g0569400) Os02t0569900-01(Os02g0569900) Os06t0599200-01(Os06g0599200)

OGL:	127763670 127764059

Reference

1 [PMID:19825834]

Authors

Swaminathan S, Morrone D, Wang Q, Fulton DB, Peters RJ

Title

CYP76M7 is an ent-cassadiene C11alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice.

Journal

Plant Cell 21:3315-25 (2009)
DOI:10.1105/tpc.108.063677

Sequence

[osa:4329723]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.112

IUBMB Enzyme Nomenclature:

1.14.14.112

ExPASy - ENZYME nomenclature database:

1.14.14.112

BRENDA, the Enzyme Database:

1.14.14.112

DBGET integrated database retrieval system