KEGG   ENZYME: 1.14.14.117
Entry
EC 1.14.14.117              Enzyme                                 
Name
aflatoxin B synthase;
ordA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
8-O-methylsterigmatocystin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (aflatoxin-B-forming)
Reaction(IUBMB)
(1) 8-O-methylsterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B1 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:R10319];
(2) 8-O-methyldihydrosterigmatocystin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = aflatoxin B2 + 2 [oxidized NADPH---hemoprotein reductase] + H2O + methanol + CO2 [RN:R10320]
Reaction(KEGG)
R10319 R10320
Substrate
8-O-methylsterigmatocystin [CPD:C03686];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
8-O-methyldihydrosterigmatocystin [CPD:C03944]
Product
aflatoxin B1 [CPD:C06800];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
methanol [CPD:C00132];
CO2 [CPD:C00011];
aflatoxin B2 [CPD:C16753]
Comment
A cytochrome P-450 (heme-thiolate) protein. Isolated from the mold Aspergillus parasiticus.
History
EC 1.14.14.117 created 2013 as EC 1.14.13.175, transferred 2018 to EC 1.14.14.117
Pathway
ec00254  Aflatoxin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K17651  aflatoxin B synthase
Genes
AOR: AO090026000027
AFV: AFLA_006290
APUU: APUU_50251A
Reference
1  [PMID:1902378]
  Authors
Bhatnagar D, Cleveland TE, Kingston DG
  Title
Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis.
  Journal
Biochemistry 30:4343-50 (1991)
DOI:10.1021/bi00231a033
Reference
2  [PMID:9835571]
  Authors
Yu J, Chang PK, Ehrlich KC, Cary JW, Montalbano B, Dyer JM, Bhatnagar D, Cleveland TE
  Title
Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2.
  Journal
Appl Environ Microbiol 64:4834-41 (1998)
DOI:10.1128/AEM.64.12.4834-4841.1998
  Sequence
[ag:AAS66031]
Reference
3  [PMID:11996570]
  Authors
Udwary DW, Casillas LK, Townsend CA
  Title
Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis.
  Journal
J Am Chem Soc 124:5294-303 (2002)
DOI:10.1021/ja012185v
  Sequence
[ag:AAS66031]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.117
IUBMB Enzyme Nomenclature: 1.14.14.117
ExPASy - ENZYME nomenclature database: 1.14.14.117
BRENDA, the Enzyme Database: 1.14.14.117

  All links  
Pathway (7)   
   KEGG PATHWAY (6)   
   KEGG MODULE (1)   
Chemical substance (10)   
   KEGG COMPOUND (10)   
Chemical reaction (3)   
   KEGG REACTION (2)   
   KEGG RCLASS (1)   
Gene (5)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (4)   
Protein sequence (8)   
   UniProt (4)   
   SWISS-PROT (4)   
Protein domain (5)   
   InterPro (5)   
All databases (38)   

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