KEGG ENZYME: 1.14.14.120

ENZYME: 1.14.14.120

Entry

EC 1.14.14.120              Enzyme

Name

dammarenediol 12-hydroxylase;
protopanaxadiol synthase;
CYP716A47

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

dammarenediol-II,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12beta-hydroxylating)

Reaction(IUBMB)

dammarenediol-II + [reduced NADPH---hemoprotein reductase] + O2 = protopanaxadiol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10540]

Reaction(KEGG)

R10540

Substrate

dammarenediol-II [CPD:C19829];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

protopanaxadiol [CPD:C20715];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein isolated from ginseng (Panax ginseng). Involved in the biosynthetic pathway of ginsenosides.

History

EC 1.14.14.120 created 2013 as EC 1.14.13.183, transferred 2018 to EC 1.14.14.120

Pathway

ec00999

Biosynthesis of various plant secondary metabolites

ec01110

Biosynthesis of secondary metabolites

Orthology

K20545

dammarenediol 12-hydroxylase

Reference

1 [PMID:22039120]

Authors

Han JY, Kim HJ, Kwon YS, Choi YE

Title

The Cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng.

Journal

Plant Cell Physiol 52:2062-73 (2011)
DOI:10.1093/pcp/pcr150

Sequence

[ag:AEY75212]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.120

IUBMB Enzyme Nomenclature:

1.14.14.120

ExPASy - ENZYME nomenclature database:

1.14.14.120

BRENDA, the Enzyme Database:

1.14.14.120

DBGET integrated database retrieval system