KEGG   ENZYME: 1.14.14.121
Entry
EC 1.14.14.121              Enzyme                                 
Name
protopanaxadiol 6-hydroxylase;
protopanaxatriol synthase;
P6H;
CYP716A53v2
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
protopanaxadiol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6alpha-hydroxylating)
Reaction(IUBMB)
protopanaxadiol + [reduced NADPH---hemoprotein reductase] + O2 = protopanaxatriol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10591]
Reaction(KEGG)
R10591
Substrate
protopanaxadiol [CPD:C20715];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
protopanaxatriol [CPD:C20716];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the rhizomes of ginseng (Panax ginseng). Involved in the biosynthetic pathway of ginsenosides.
History
EC 1.14.14.121 created 2013 as EC 1.14.13.184, transferred 2018 to EC 1.14.14.121
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01110  Biosynthesis of secondary metabolites
Orthology
K20546  protopanaxadiol 6-hydroxylase
Reference
1  [PMID:18351680]
  Authors
Yue CJ, Zhou X, Zhong JJ
  Title
Protopanaxadiol 6-hydroxylase and its role in regulating the ginsenoside heterogeneity in Panax notoginseng cells.
  Journal
Biotechnol Bioeng 100:933-40 (2008)
DOI:10.1002/bit.21829
Reference
2  [PMID:22875608]
  Authors
Han JY, Hwang HS, Choi SW, Kim HJ, Choi YE
  Title
Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng.
  Journal
Plant Cell Physiol 53:1535-45 (2012)
DOI:10.1093/pcp/pcs106
  Sequence
[ag:AFO63031]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.121
IUBMB Enzyme Nomenclature: 1.14.14.121
ExPASy - ENZYME nomenclature database: 1.14.14.121
BRENDA, the Enzyme Database: 1.14.14.121

  All links  
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (14)   
   UniProt (13)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (32)   

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