KEGG ENZYME: 1.14.14.123

ENZYME: 1.14.14.123

Entry

EC 1.14.14.123              Enzyme

Name

oryzalexin D synthase;
CYP76M8

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

ent-sandaracopimaradien-3beta-ol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (oryzalexin-D-forming)

Reaction(IUBMB)

ent-sandaracopimaradien-3beta-ol + [reduced NADPH---hemoprotein reductase] + O2 = oryzalexin D + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11658]

Reaction(KEGG)

R11658

Substrate

ent-sandaracopimaradien-3beta-ol [CPD:C21560];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

oryzalexin D [CPD:C21562];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein. Isolated from Oryza sativa (rice). Oryzalexin D is a phytoalexin.

History

EC 1.14.14.123 created 2014 as EC 1.14.13.193, transferred 2018 to EC 1.14.14.123

Pathway

ec00904

Diterpenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K16084

ent-cassa-12,15-diene 11-hydroxylase / oryzalexin D synthase

Genes

OSA:	4329722 4329723 4341447

DOSA:

Os02t0569400-01(Os02g0569400) Os02t0569900-01(Os02g0569900) Os06t0599200-01(Os06g0599200)

OGL:	127763670 127764059

Reference

1 [PMID:23795884]

Authors

Wu Y, Wang Q, Hillwig ML, Peters RJ

Title

Picking sides: distinct roles for CYP76M6 and CYP76M8 in rice oryzalexin biosynthesis.

Journal

Biochem J 454:209-16 (2013)
DOI:10.1042/BJ20130574

Sequence

[osa:4329722]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.123

IUBMB Enzyme Nomenclature:

1.14.14.123

ExPASy - ENZYME nomenclature database:

1.14.14.123

BRENDA, the Enzyme Database:

1.14.14.123

DBGET integrated database retrieval system