KEGG ENZYME: 1.14.14.13

ENZYME: 1.14.14.13

Entry

EC 1.14.14.13               Enzyme

Name

4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] monooxygenase;
btrO (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein],FMNH2:oxygen oxidoreductase (2-hydroxylating)

Reaction(IUBMB)

4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2 = 4-(gamma-L-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O [RN:R08908]

Reaction(KEGG)

R08908

Substrate

4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein];
FMNH2 [CPD:C01847];
O2 [CPD:C00007]

Product

4-(gamma-L-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein];
FMN [CPD:C00061];
H2O [CPD:C00001]

Comment

Catalyses a step in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. FMNH2 is used as a free cofactor. Forms a complex with a dedicated NAD(P)H:FMN oxidoreductase. The enzyme is not able to hydroxylate free substrates, activation by the acyl-carrier protein is mandatory.
Octanoyl-S-[BtrI acyl-carrier protein] is also accepted.

History

EC 1.14.14.13 created 2012

Pathway

ec00524

Neomycin, kanamycin and gentamicin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13561

4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] monooxygenase

Reference

1 [PMID:15975512]