KEGG ENZYME: 1.14.14.130

ENZYME: 1.14.14.130

Entry

EC 1.14.14.130              Enzyme

Name

laurate 7-monooxygenase;
CYP703A2 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

dodecanoate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (7-hydroxylating)

Reaction(IUBMB)

dodecanoate + [reduced NADPH---hemoprotein reductase] + O2 = 7-hydroxydodecanoate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11054]

Reaction(KEGG)

R11054

Substrate

dodecanoate [CPD:C02679];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

7-hydroxydodecanoate [CPD:C21080];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein found in plants. The enzyme is involved in the synthesis of sporopollenin - a complex polymer found at the outer layer of spores and pollen. It can also act on decanoate (C10), myristate (C14), and palmitate (C16) with lower activity. The enzyme also produces a small amount of products that are hydroxylated at neighboring positions (C-6, C-8 and C-9).

History

EC 1.14.14.130 created 2015 as EC 1.14.13.206, transferred 2018 to EC 1.14.14.130

Orthology

K20496

laurate 7-monooxygenase

Genes

ATH:	AT1G01280(CYP703A2)

ALY:

9328138

CRB:

17896796

CSAT:

104738665 104754273 104778519

EUS:	EUTSA_v10007395mg

BRP:

103844638

BNA:	106370068 106400415

BOE:

106293335

RSZ:	108853661 130507937

THJ:

104825880

CPAP:

110811031

CIT:	102625867 127901300

CIC:	CICLE_v10023470mg

PVY:	116121534 116121561

MINC:

123222697

TCC:

18613966

GRA:

105762440

GHI:	107947528 107949243

GAB:

108477801

HSYR:

120205590

DZI:

111305969

EGR:

104443225

GMX:

100789889

GSJ:

114378926

PVU:	PHAVU_011G159600g

VRA:

106752700

VAR:

108339159

VUN:	114170177 114170386

VUM:

124842895

CCAJ:

109806212

APRC:

113865136

MTR:

11438480

TPRA:

123889409

CAM:

101514759

PSAT:

127126544

VVO:

131623646

LJA:	Lj3g3v0428480.1(Lj3g3v0428480.1)

ADU:

107478471

AIP:

107634316

AHF:	112737919 112790061

LANG:

109363395

PCIN:

129287495

FVE:

101293350

RCN:

112169376

PPER:

18787141

PMUM:

103332232

PAVI:

110757680

PDUL:

117618316

MDM:

114823871

MSYL:

126614013

PXB:

103929347

ZJU:

107423645

MNT:

21406259

CSAV:

115707784

CSV:

101205312

CMO:

103483180

BHJ:

120070266

MCHA:

111016260

CMAX:

111479555

CMOS:

111431947

CPEP:

111790097

RCU:

8282130

JCU:

105642840

HBR:

110653613

MESC:

110614660

POP:

7497255

PEU:

105124757

PALZ:

118063450

JRE:

108989252

CILL:

122311318

CAVE:

132187184

QSU:

111989342

QLO:

115983998

TWL:

119981982

VVI:

100253484

VRI:

117932706

SLY:

101265072

SPEN:

107032022

SOT:

102601340

SSTN:

125865613

SDUL:

129876734

CANN:

107845939

LBB:

132630107

NTA:	107788932 107814441

NSY:

104217226

NTO:

104085251

NAU:

109231543

INI:

109155042

ITR:

115995652

SIND:

105173745

OEU:	111411946 111411948

EGT:

105963913

SSPL:

121751700 121792397

SMIL:

130997352

SHIS:

125217837

APAN:

127255154

HAN:

110863788

ECAD:

122590472

LSV:

111894621

CCAV:

112526455

DCR:

108208274

CSIN:

114323145

RVL:

131307178

AEW:

130780599

BVG:

104882800

SOE:

110785044

CQI:	110713969 110725131

ATRI:

130809629

MOF:

131165558

NNU:

104610254

MING:

122065199

TSS:

122639703

PSOM:

113286310 113332658

OSA:

4344594

DOSA:

Os08t0131100-01(Os08g0131100)

OBR:

102704827

OGL:

127783286

BDI:

112268508

TDC:	119331949 119340947

TAES:

123147446 123157915 123165638

TUA:

125521741

LPER:

127314439

LRD:	124682634 124683186

SBI:

8074081

ZMA:

100282873

SITA:

101785968

SVS:

117860598

PVIR:

120677639 120711322

PHAI:

112898024

PDA:

120107104

EGU:

105059962

MUS:

103995628

ZOF:	121980268 121980270 121980407 121980409 121980410 121984178 121984179 121984180 121984181 121984312

DCT:

110114706

PEQ:

110026608

AOF:

109844353

MSIN:

131221718

NCOL:

116264350

ATR:

18427181

SMO:	SELMODRAFT_144382(CYP703C2_v2) SELMODRAFT_168652(CYP703C1_v2) SELMODRAFT_229947(CYP703C1) SELMODRAFT_74166(CYP703C2)

PPP:

112284522

» show all

Reference

1 [PMID:17496121]

Authors

Morant M, Jorgensen K, Schaller H, Pinot F, Moller BL, Werck-Reichhart D, Bak S

Title

CYP703 is an ancient cytochrome P450 in land plants catalyzing in-chain hydroxylation of lauric acid to provide building blocks for sporopollenin synthesis in pollen.

Journal

Plant Cell 19:1473-87 (2007)
DOI:10.1105/tpc.106.045948

Sequence

[ath:AT1G01280]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.130

IUBMB Enzyme Nomenclature:

1.14.14.130

ExPASy - ENZYME nomenclature database:

1.14.14.130

BRENDA, the Enzyme Database:

1.14.14.130

DBGET integrated database retrieval system