KEGG   ENZYME: 1.14.14.131
Entry
EC 1.14.14.131              Enzyme                                 
Name
bursehernin 5'-monooxygenase;
CYP71CU1 (gene name);
bursehernin 5'-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(-)-bursehernin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Reaction(IUBMB)
(-)-bursehernin + [reduced NADPH---hemoprotein reductase] + O2 = (-)-5'-demethylyatein + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11211]
Reaction(KEGG)
R11211
Substrate
(-)-bursehernin [CPD:C21183];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-5'-demethylyatein [CPD:C21184];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Sinopodophyllum hexandrum. The enzyme is involved in the biosynthetic pathway of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 1.14.14.131 created 2016 as EC 1.14.13.213, transferred 2018 to EC 1.14.14.131
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21205  bursehernin 5'-monooxygenase
Reference
1  [PMID:26359402]
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.131
IUBMB Enzyme Nomenclature: 1.14.14.131
ExPASy - ENZYME nomenclature database: 1.14.14.131
BRENDA, the Enzyme Database: 1.14.14.131

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